The positional capture experiments of the LCM using near field fiber probe

Chi Fu Yen, Jen Ai Lee, Chien Ming Chen

研究成果: 書貢獻/報告類型會議貢獻

摘要

Laser Capture Microdissection (LCM) is a technique that permits rapid and reliable procurement of pure population of cells from tissue sections. We created the LCM system with a near field fiber probe to transmit the laser light (808 nm) to heat the thermoplastic polymer film which was placed above the tissue section. Laser spots in nano-dimension cause the film to be melted and fused with the underlying target of choice. In the study, we set the fiber probe on a two-dimensional nanometer PZT to do precisely capture. The aperture of fiber probe we used is 100 nm through which light can be focused. We used 20 nm spread gold particles to be captured in these experiments instead of tissue sections. By moving the probe in X direction to melt spots in different distances, we can know more molecular properties of the thermoplastic polymer film especially in its resolving power. When the film is removed, the chosen particles remain bound to the film, while the rest of the tissue or particles are left behind. According to the results of this study, we can know how close the melted spots can reach to, and it is also helpful to the operation of this new LCM system.
原文英語
主出版物標題Proceedings of SPIE - The International Society for Optical Engineering
編輯C. Quan, F.S. Chau, A. Asundi, B.S. Wong, C.T. Lim
頁面844-848
頁數5
5852 PART II
DOIs
出版狀態已發佈 - 2005
事件Third International Conference on Experimental Mechanics and Third Conference of the Asian Committee on Experimental Mechanics - Singapore, 新加坡
持續時間: 十一月 29 2004十二月 1 2004

其他

其他Third International Conference on Experimental Mechanics and Third Conference of the Asian Committee on Experimental Mechanics
國家/地區新加坡
城市Singapore
期間11/29/0412/1/04

ASJC Scopus subject areas

  • 電氣與電子工程
  • 凝聚態物理學

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