TY - JOUR
T1 - The phenolic constituent and free radical scavenging activities of Gynura formosona Kiamnra
AU - Hou, Wen Chi
AU - Lin, Rong Dih
AU - Li, Zong-Huei
AU - Huang, Ying Hua
AU - Hsu, Feng-Lin
AU - Lee, Mei Hsien
PY - 2005/3
Y1 - 2005/3
N2 - Gynura formosana Kiamnra (Compositae) is a herbal folk medicine that is a popular vegetable in Taiwan. The free-radical scavenging activities of a 70% aqueous acetone extract from the herb G formosana were evaluated. Bioassay-guided fractionation, column separation on Diaion, Toyopearl HW 40(C), Sephadex LH-20 and MCI CHP20P, and high-performance liquid chromatography (HPLC) were used to isolate for the first time in G formosana four potent phenolics [caffeic acid (I), quercetin 3-O-rutinoside (II), kaempferol 3-O-rutinoside (III) and kaempferol 3-O-robinobioside (IV)]. The IC50 values of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity for compounds I-IV were 6.7, 7.7, 300.3 and 286.7 μM, respectively, and, for superoxide radical scavenging activity, they were 187.3, 25.8, 55.3 and 87.4 μM, respectively. Using a spin trapping electron spin resonance (ESR) method, caffeic acid (I) and quercetin 3-O-rutinoside (II) exhibited good hydroxyl radical activity. The free radical scavenging activity of G formosana phenolics may improve the economic value of this herb and assist in its development as a health food.
AB - Gynura formosana Kiamnra (Compositae) is a herbal folk medicine that is a popular vegetable in Taiwan. The free-radical scavenging activities of a 70% aqueous acetone extract from the herb G formosana were evaluated. Bioassay-guided fractionation, column separation on Diaion, Toyopearl HW 40(C), Sephadex LH-20 and MCI CHP20P, and high-performance liquid chromatography (HPLC) were used to isolate for the first time in G formosana four potent phenolics [caffeic acid (I), quercetin 3-O-rutinoside (II), kaempferol 3-O-rutinoside (III) and kaempferol 3-O-robinobioside (IV)]. The IC50 values of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity for compounds I-IV were 6.7, 7.7, 300.3 and 286.7 μM, respectively, and, for superoxide radical scavenging activity, they were 187.3, 25.8, 55.3 and 87.4 μM, respectively. Using a spin trapping electron spin resonance (ESR) method, caffeic acid (I) and quercetin 3-O-rutinoside (II) exhibited good hydroxyl radical activity. The free radical scavenging activity of G formosana phenolics may improve the economic value of this herb and assist in its development as a health food.
KW - Caffeic acid
KW - Free-radical scavenging
KW - Gynura formosana
KW - Kaempferol 3-O-robinobioside
KW - Kaempferol 3-O-rutinoside
KW - Quercetin 3-O-rutinoside
UR - http://www.scopus.com/inward/record.url?scp=14144249480&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=14144249480&partnerID=8YFLogxK
U2 - 10.1002/jsfa.2017
DO - 10.1002/jsfa.2017
M3 - Article
AN - SCOPUS:14144249480
VL - 85
SP - 615
EP - 621
JO - Journal of the Science of Food and Agriculture
JF - Journal of the Science of Food and Agriculture
SN - 0022-5142
IS - 4
ER -