The neuroprotective effects of an extract of Gastrodia elata

Chung Fen Tsai, Chuen Lin Huang, Yun Lian Lin, Yi Chao Lee, Ying Chen Yang, Nai Kuei Huang

研究成果: 雜誌貢獻文章

31 引文 (Scopus)

摘要

Ethnopharmacological relevance: Gastrodia elata (GE) Blume (family Orchidaceae) is a traditional Chinese herbal medicine for treating headaches, dizziness, tetanus, and epilepsy, indicating neuronal protective functions. Aim of the study: To evaluate the neuroprotection of GE and its molecular mechanism in preventing serum deprivation-induced PC12 cell apoptosis. Materials and methods: An MTT assay and Hoechst staining were used to respectively validate serum deprivation-induced cell death and apoptosis. Cyclic (c)AMP formation and protein kinase (PK)A activity were also measured after GE treatment. Western blotting was used to detect the phosphorylation of the cAMP response element-binding (CREB) protein. Transient transfection of a dominant negative CREB was used to validate the importance of CREB. Results: GE targeted the adenosine A 2A receptor (A 2A-R). GE increased cAMP formation, PKA activity, and phosphorylation of the CREB protein. GE-induced CREB protein phosphorylation and protection was blocked by a PKA inhibitor and overexpression of the dominant negative CREB, respectively. Conclusions: These results support the neuroprotective effects of GE. The protective mechanism might be mediated through an A 2A-R/cAMP/PKA/CREB-dependent pathway.

原文英語
頁(從 - 到)119-125
頁數7
期刊Journal of Ethnopharmacology
138
發行號1
DOIs
出版狀態已發佈 - 十月 31 2011
對外發佈Yes

指紋

Gastrodia
Neuroprotective Agents
Response Elements
Cyclic AMP Response Element-Binding Protein
Phosphorylation
Orchidaceae
Cyclic AMP Receptors
Adenosine A2A Receptors
Apoptosis
Adenylate Kinase
Herbal Medicine
PC12 Cells
Tetanus
Dizziness
Cyclic AMP-Dependent Protein Kinases
Serum
Cyclic AMP
Transfection
Headache
Epilepsy

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

引用此文

Tsai, C. F., Huang, C. L., Lin, Y. L., Lee, Y. C., Yang, Y. C., & Huang, N. K. (2011). The neuroprotective effects of an extract of Gastrodia elata. Journal of Ethnopharmacology, 138(1), 119-125. https://doi.org/10.1016/j.jep.2011.08.064

The neuroprotective effects of an extract of Gastrodia elata. / Tsai, Chung Fen; Huang, Chuen Lin; Lin, Yun Lian; Lee, Yi Chao; Yang, Ying Chen; Huang, Nai Kuei.

於: Journal of Ethnopharmacology, 卷 138, 編號 1, 31.10.2011, p. 119-125.

研究成果: 雜誌貢獻文章

Tsai, CF, Huang, CL, Lin, YL, Lee, YC, Yang, YC & Huang, NK 2011, 'The neuroprotective effects of an extract of Gastrodia elata', Journal of Ethnopharmacology, 卷 138, 編號 1, 頁 119-125. https://doi.org/10.1016/j.jep.2011.08.064
Tsai, Chung Fen ; Huang, Chuen Lin ; Lin, Yun Lian ; Lee, Yi Chao ; Yang, Ying Chen ; Huang, Nai Kuei. / The neuroprotective effects of an extract of Gastrodia elata. 於: Journal of Ethnopharmacology. 2011 ; 卷 138, 編號 1. 頁 119-125.
@article{a8958dbc4e3b49158c5c6f642e70ead5,
title = "The neuroprotective effects of an extract of Gastrodia elata",
abstract = "Ethnopharmacological relevance: Gastrodia elata (GE) Blume (family Orchidaceae) is a traditional Chinese herbal medicine for treating headaches, dizziness, tetanus, and epilepsy, indicating neuronal protective functions. Aim of the study: To evaluate the neuroprotection of GE and its molecular mechanism in preventing serum deprivation-induced PC12 cell apoptosis. Materials and methods: An MTT assay and Hoechst staining were used to respectively validate serum deprivation-induced cell death and apoptosis. Cyclic (c)AMP formation and protein kinase (PK)A activity were also measured after GE treatment. Western blotting was used to detect the phosphorylation of the cAMP response element-binding (CREB) protein. Transient transfection of a dominant negative CREB was used to validate the importance of CREB. Results: GE targeted the adenosine A 2A receptor (A 2A-R). GE increased cAMP formation, PKA activity, and phosphorylation of the CREB protein. GE-induced CREB protein phosphorylation and protection was blocked by a PKA inhibitor and overexpression of the dominant negative CREB, respectively. Conclusions: These results support the neuroprotective effects of GE. The protective mechanism might be mediated through an A 2A-R/cAMP/PKA/CREB-dependent pathway.",
keywords = "A -R, Apoptosis, cAMP, CREB, Gastrodia elata, Pheochromocytoma cells, PKA",
author = "Tsai, {Chung Fen} and Huang, {Chuen Lin} and Lin, {Yun Lian} and Lee, {Yi Chao} and Yang, {Ying Chen} and Huang, {Nai Kuei}",
year = "2011",
month = "10",
day = "31",
doi = "10.1016/j.jep.2011.08.064",
language = "English",
volume = "138",
pages = "119--125",
journal = "Journal of Ethnopharmacology",
issn = "0378-8741",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - The neuroprotective effects of an extract of Gastrodia elata

AU - Tsai, Chung Fen

AU - Huang, Chuen Lin

AU - Lin, Yun Lian

AU - Lee, Yi Chao

AU - Yang, Ying Chen

AU - Huang, Nai Kuei

PY - 2011/10/31

Y1 - 2011/10/31

N2 - Ethnopharmacological relevance: Gastrodia elata (GE) Blume (family Orchidaceae) is a traditional Chinese herbal medicine for treating headaches, dizziness, tetanus, and epilepsy, indicating neuronal protective functions. Aim of the study: To evaluate the neuroprotection of GE and its molecular mechanism in preventing serum deprivation-induced PC12 cell apoptosis. Materials and methods: An MTT assay and Hoechst staining were used to respectively validate serum deprivation-induced cell death and apoptosis. Cyclic (c)AMP formation and protein kinase (PK)A activity were also measured after GE treatment. Western blotting was used to detect the phosphorylation of the cAMP response element-binding (CREB) protein. Transient transfection of a dominant negative CREB was used to validate the importance of CREB. Results: GE targeted the adenosine A 2A receptor (A 2A-R). GE increased cAMP formation, PKA activity, and phosphorylation of the CREB protein. GE-induced CREB protein phosphorylation and protection was blocked by a PKA inhibitor and overexpression of the dominant negative CREB, respectively. Conclusions: These results support the neuroprotective effects of GE. The protective mechanism might be mediated through an A 2A-R/cAMP/PKA/CREB-dependent pathway.

AB - Ethnopharmacological relevance: Gastrodia elata (GE) Blume (family Orchidaceae) is a traditional Chinese herbal medicine for treating headaches, dizziness, tetanus, and epilepsy, indicating neuronal protective functions. Aim of the study: To evaluate the neuroprotection of GE and its molecular mechanism in preventing serum deprivation-induced PC12 cell apoptosis. Materials and methods: An MTT assay and Hoechst staining were used to respectively validate serum deprivation-induced cell death and apoptosis. Cyclic (c)AMP formation and protein kinase (PK)A activity were also measured after GE treatment. Western blotting was used to detect the phosphorylation of the cAMP response element-binding (CREB) protein. Transient transfection of a dominant negative CREB was used to validate the importance of CREB. Results: GE targeted the adenosine A 2A receptor (A 2A-R). GE increased cAMP formation, PKA activity, and phosphorylation of the CREB protein. GE-induced CREB protein phosphorylation and protection was blocked by a PKA inhibitor and overexpression of the dominant negative CREB, respectively. Conclusions: These results support the neuroprotective effects of GE. The protective mechanism might be mediated through an A 2A-R/cAMP/PKA/CREB-dependent pathway.

KW - A -R

KW - Apoptosis

KW - cAMP

KW - CREB

KW - Gastrodia elata

KW - Pheochromocytoma cells

KW - PKA

UR - http://www.scopus.com/inward/record.url?scp=80255133201&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80255133201&partnerID=8YFLogxK

U2 - 10.1016/j.jep.2011.08.064

DO - 10.1016/j.jep.2011.08.064

M3 - Article

C2 - 21925258

AN - SCOPUS:80255133201

VL - 138

SP - 119

EP - 125

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

SN - 0378-8741

IS - 1

ER -