摘要
In order to dissect out whether multiple activities of cardiotoxins (CTXs) are connected, to some extent, with each other, studies on reduced and S-carboxyamidomethylated (Rcam) Naja naja atra CTX3 were carried out in the present study. Although both CTX3 and Rcam-CTX3 induced apoptotic death of PC-3 cells as evidenced by propodium iodide/annexin V double staining, degradation of procaspases and DNA fragmentation, the cytotoxicity of Rcam-CTX3 was mostly 100-fold lower than that noted with native toxin. However, Rcam-CTX3 retained approximately 38% of the membrane-damaging activity of native toxin as revealed by the decrease in calcein self-quenching from phospholipid vesicles. These results are likely to reflect that the mechanism of cytotoxicity by CTX3 is not heavily dependent on its membrane-perturbing effect, and suggest that the structural elements within CTX3 responsible for the two activities are probably separated.
原文 | 英語 |
---|---|
頁(從 - 到) | 816-824 |
頁數 | 9 |
期刊 | Toxicon |
卷 | 50 |
發行號 | 6 |
DOIs | |
出版狀態 | 已發佈 - 11月 2007 |
對外發佈 | 是 |
ASJC Scopus subject areas
- 毒理學