The limited deglucosylation process of β-glucosidase in Bacillus cereus H62L for biotransforming secoisolariciresinol diglucoside into mammalian lignans

Chin Fa Hwang, Hui Er Wang, Yaw Bee Ker, Chiung Chi Peng, Kuan Chou Chen, Robert Y. Peng

研究成果: 雜誌貢獻文章同行評審

1 引文 斯高帕斯(Scopus)

摘要

The amount of major lignans present in the alkaline extract of the defatted flaxseed (in mg/g) is as follows: secoisolariciresinol diglucoside (SDG, 109.80), p-coumaric acid glucoside (CAG, 70.21), ferulic acid glucoside (FAG, 68.81), secoisolariciresinol (SECO, 0.49), coumaric acid (CA, 0.37), and ferulic acid (FA, 0.85). When biotransformed, SDG yields two valuable mammalian lignans (MLG): enterodiol (END) and enterolactone (ENL). Bacillus cereus strain H62-L1 was isolated and tested for the process of bioconversion. Kinetic analysis revealed the sequential reaction SDG (A) → CAG (B) → END (C) → ENL (D) to be reversible at the first step with the generalized reaction type Ak2k1Bk3Ck4D. The obtained rate coefficients were as follows: k1 = 0.001-0.028 h-1, k 2 = 0.006-0.077 h-1, k3 = 0.013 h-1, and k4 = 0.002 h-1, and the overall kinetic parameter k1/(k2 + k3) varied from 0.05 to 1.32, mostly not favoring the forward deglucosylation reaction. SDG with higher purity favored the forward reaction. In conclusion, to facilitate the production rate, we propose (i) using substrate SDG with high purity at a higher concentration; (ii) using an allosteric inhibitor to block the reverse reaction from SECO to SDG; or (iii) genetically modifying Bacillus cereus H62-L1.
原文英語
頁(從 - 到)1632-1640
頁數9
期刊Process Biochemistry
46
發行號8
DOIs
出版狀態已發佈 - 八月 2011

ASJC Scopus subject areas

  • 生物工程
  • 生物化學
  • 應用微生物與生物技術

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