The Involvement of Active DNA Synthesis in Camptothecin-induced G2 Arrest: Altered Regulation of p34cdc2/Cyclin B

Yeou Ping Tsao, Peter D'Arpa, Leroy F. Liu

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229 引文 斯高帕斯(Scopus)

摘要

Cell cycle arrest in G2 phase is a common response to a variety of DNA-damaging agents. The coupling between DNA damage and G2 arrest was studied in synchronized HeLa cells using camptothecin, a highly specific inhibitor of topoisomerase I that damages DNA through the formation of reversible topoisomerase I-DNA cleavable complexes. Brief camptothecin treatment of early S-phase HeLa cells caused arrest at G2 phase and abolished the activation of p34cdc2 protein kinase. Both tyrosine dephosphorylation of p34c*2 and cyclin B accumulation were altered. These cell cycle-dependent changes were not observed when DNA replication was inhibited by aphidicolin during the brief camptothecin treatment. Our results suggest that to produce G2 arrest, active DNA synthesis is required at the time of camptothecin treatment, as was previously shown for camptothecin-induced cytotoxicity. Furthermore, our results suggest that the interaction of the replication fork with DNA damage may ultimately trigger altered regulation of p34cdc2/cyclin B, leading to cell cycle arrest at the G2 phase.

原文英語
頁(從 - 到)1823-1829
頁數7
期刊Cancer Research
52
發行號7
出版狀態已發佈 - 四月 1992

ASJC Scopus subject areas

  • 腫瘤科
  • 癌症研究

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