摘要
Many diseases occur when the immune system is weakened. Intracellular signals activate immuno-responsive cells to produce cytokines that modulate the immune response. Schisandra chinensis has been used traditionally to treat general fatigue, neurasthenia, and spontaneous sweating. In the present study, the effect of constituents of S. chinensis on cytokine release by human monocytic leukemia cells (THP-1) was tested using microparticle-based flow cytometric analysis. Two major lignans, schizandrin (Sch) and gomisin A (Gom A), were identified and shown to induce interleukin (IL)-8, macrophage inflammatory protein-1β (MIP-1β), and granulocyte-macrophage-colony stimulating factor (GM-CSF) release by THP-1 cells. By reverse transcription polymerase chain reaction (RT-PCR) or quantitative real-time PCR, there was a dose-dependent increase of IL-8, MIP-1β and GM-CSF mRNA levels. Thus, Sch and Gom A from S. chinensis enhance cytokine release by THP-1 cells and this effect occurs through mRNA upregulation. Upregulation of MIP-1β and GM-CSF in particular may have clinical applications. Therefore, S. chinensis may be therapeutically beneficial by promoting humoral and cell-mediated immune responses.
| 原文 | 英語 |
|---|---|
| 頁（從 - 到） | 4836-4849 |
| 頁數 | 14 |
| 期刊 | Molecules |
| 卷 | 16 |
| 發行號 | 6 |
| DOIs | |
| 出版狀態 | 已發佈 - 六月 2011 |
指紋
ASJC Scopus subject areas
- Organic Chemistry
引用此文
The immuno-regulatory effects of Schisandra chinensis and its constituents on human monocytic leukemia cells. / Lin, Rong Dih; Mao, Yi Wen; Leu, Sy Jye; Huang, Ching Yi; Lee, Mei Hsien.
於: Molecules, 卷 16, 編號 6, 06.2011, p. 4836-4849.研究成果: 雜誌貢獻 › 文章
}
TY - JOUR
T1 - The immuno-regulatory effects of Schisandra chinensis and its constituents on human monocytic leukemia cells
AU - Lin, Rong Dih
AU - Mao, Yi Wen
AU - Leu, Sy Jye
AU - Huang, Ching Yi
AU - Lee, Mei Hsien
PY - 2011/6
Y1 - 2011/6
N2 - Many diseases occur when the immune system is weakened. Intracellular signals activate immuno-responsive cells to produce cytokines that modulate the immune response. Schisandra chinensis has been used traditionally to treat general fatigue, neurasthenia, and spontaneous sweating. In the present study, the effect of constituents of S. chinensis on cytokine release by human monocytic leukemia cells (THP-1) was tested using microparticle-based flow cytometric analysis. Two major lignans, schizandrin (Sch) and gomisin A (Gom A), were identified and shown to induce interleukin (IL)-8, macrophage inflammatory protein-1β (MIP-1β), and granulocyte-macrophage-colony stimulating factor (GM-CSF) release by THP-1 cells. By reverse transcription polymerase chain reaction (RT-PCR) or quantitative real-time PCR, there was a dose-dependent increase of IL-8, MIP-1β and GM-CSF mRNA levels. Thus, Sch and Gom A from S. chinensis enhance cytokine release by THP-1 cells and this effect occurs through mRNA upregulation. Upregulation of MIP-1β and GM-CSF in particular may have clinical applications. Therefore, S. chinensis may be therapeutically beneficial by promoting humoral and cell-mediated immune responses.
AB - Many diseases occur when the immune system is weakened. Intracellular signals activate immuno-responsive cells to produce cytokines that modulate the immune response. Schisandra chinensis has been used traditionally to treat general fatigue, neurasthenia, and spontaneous sweating. In the present study, the effect of constituents of S. chinensis on cytokine release by human monocytic leukemia cells (THP-1) was tested using microparticle-based flow cytometric analysis. Two major lignans, schizandrin (Sch) and gomisin A (Gom A), were identified and shown to induce interleukin (IL)-8, macrophage inflammatory protein-1β (MIP-1β), and granulocyte-macrophage-colony stimulating factor (GM-CSF) release by THP-1 cells. By reverse transcription polymerase chain reaction (RT-PCR) or quantitative real-time PCR, there was a dose-dependent increase of IL-8, MIP-1β and GM-CSF mRNA levels. Thus, Sch and Gom A from S. chinensis enhance cytokine release by THP-1 cells and this effect occurs through mRNA upregulation. Upregulation of MIP-1β and GM-CSF in particular may have clinical applications. Therefore, S. chinensis may be therapeutically beneficial by promoting humoral and cell-mediated immune responses.
KW - Gomisin A
KW - Granulocyte-macrophage-colony stimulating factor
KW - Macrophage inflammatory protein-1β
KW - Polymerase chain reaction
KW - Schizandrin
UR - http://www.scopus.com/inward/record.url?scp=79959602727&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79959602727&partnerID=8YFLogxK
U2 - 10.3390/molecules16064836
DO - 10.3390/molecules16064836
M3 - Article
VL - 16
SP - 4836
EP - 4849
JO - Molecules
JF - Molecules
SN - 1420-3049
IS - 6
ER -