The high salt form of poly(dG-dC)·poly(dG-dC) is left-handed Z-DNA: Raman spectra of crystals and solutions

Thomas J. Thamann, Richard C. Lord, Andrew H.J. Wang, Alexander Rich

研究成果: 雜誌貢獻文章同行評審

237 引文 斯高帕斯(Scopus)

摘要

The laser-Raman spectra of crystalline d(CpGpCpGpCpG) and of aqueous poly(dG-dC)·poly(dG-dC) in high salt (4M NaCl) and low salt (0.lM NaCl) solutions have been measured and compared. The spectra of the crystal and the high-salt solution show a striking congruence, which indicates clearly that the high-salt form of the aqueous polymer has the left-handed Z-DNA structure of the crystalline oligomer. These two spectra differ substantially from that of the low-salt form of the polymer, which has been found previously to have spectral characteristics of the B-form of DNA. The high salt spectrum shows a unique line due to guanine residues at 625 cm-1 which should be useful for qualitative and possibly quantitative assessment of the amount of Z-structure present in a sample of DNA.

原文英語
頁(從 - 到)5443-5458
頁數16
期刊Nucleic Acids Research
9
發行號20
DOIs
出版狀態已發佈 - 10月 24 1981
對外發佈

ASJC Scopus subject areas

  • 遺傳學

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