The functional interplay of lncRNA EGOT and HuR regulates hypoxia-induced autophagy in renal tubular cells

I. Kuan Wang, Kalaiselvi Palanisamy, Kuo Ting Sun, Shao Hua Yu, Tung Min Yu, Ching Hao Li, Feng Yen Lin, An Kuo Chou, Guei Jane Wang, Kuen Bao Chen, Chi Yuan Li

研究成果: 雜誌貢獻文章

摘要

Autophagy, an important cellular homeostatic mechanism regulates cell survival under stress and protects against acute kidney injury. However, the role of long noncoding RNA (lncRNA) in autophagy regulation in renal tubular cells (HK-2) is unclear. The study was aimed to understand the importance of lncRNA in hypoxia-induced autophagy in HK-2 cells. LncRNA eosinophil granule ontogeny transcript (EGOT) was identified as autophagy-associated lncRNA under hypoxia. The lncRNA EGOT expression was significantly downregulated in renal tubular cells during hypoxia-induced autophagy. Gain- and loss-of-EGOT functional studies revealed that EGOT overexpression reduced autophagy by downregulation of ATG7, ATG16L1, LC3II expressions and LC 3 puncta while EGOT knockdown reversed the suppression of autophagy. Importantly, RNA-binding protein, (ELAVL1)/Hu antigen R (HuR) binds and stabilizes the EGOT expression under normoxia and ATG7/16L1 expressions under hypoxia. Furthermore, HuR mediated stabilization of ATG7/16L1 expressions under hypoxia causes a decline in EGOT levels and thereby promotes autophagy. Altogether, the study first reveals the functional interplay of lncRNA EGOT and HuR on the posttranscriptional regulation of the ATG7/16L1 expressions. Thus, the HuR/EGOT/ATG7/16L1 axis is crucial for hypoxia-induced autophagy in renal tubular cells.

原文英語
期刊Journal of Cellular Biochemistry
DOIs
出版狀態接受/付印 - 一月 1 2020

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

指紋 深入研究「The functional interplay of lncRNA EGOT and HuR regulates hypoxia-induced autophagy in renal tubular cells」主題。共同形成了獨特的指紋。

  • 引用此

    Wang, I. K., Palanisamy, K., Sun, K. T., Yu, S. H., Yu, T. M., Li, C. H., Lin, F. Y., Chou, A. K., Wang, G. J., Chen, K. B., & Li, C. Y. (認可的出版社/出版中). The functional interplay of lncRNA EGOT and HuR regulates hypoxia-induced autophagy in renal tubular cells. Journal of Cellular Biochemistry. https://doi.org/10.1002/jcb.29669