The flavonoid baicalin protects against cerebrovascular dysfunction and brain inflammation in experimental heatstroke

Ching Ping Chang, Wu Tein Huang, Bor Chih Cheng, Chuan Chih Hsu, Mao Tsun Lin

研究成果: 雜誌貢獻文章

50 引文 斯高帕斯(Scopus)

摘要

The present study was performed to assess the prophylactic effect of baicalin, a flavonoid compound, in an animal model of heatstroke. Anesthetized rats, immediately before the start of heat stress, were divided into two major groups and given the following: vehicle solution (1 mL per kg body weight) or baicalin (10-40 mg per kg body weight) intravenously. They were exposed to ambient temperature of 43 °C to induce heatstroke. Another group of rats was exposed to room temperature (24 °C) and used as normothermic controls. Their physiologic and biochemical parameters were continuously monitored. When the vehicle-pretreated rats underwent heat stress, their survival time values were found to be 20-28 min. Pretreatment with intravenous doses of baicalin significantly improved survival during heatstroke (65-248 min). As compared to those of normothermic controls, all vehicle-pretreated heatstroke animals displayed higher levels of core temperature, intracranial pressure, and nitric oxide metabolite (NO2 -), glutamate, glycerol, lactate/pyruvate ratio, and dihydroxybenzoic acid (DHBA) in hypothalamus. In addition, both serum and hypothalamic levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) as well as plasma levels of creatinine, serum urea nitrogen, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase and alkaline phosphatase were elevated after heatstroke onset. In contrast, all vehicle-pretreated heatstroke animals had lower levels of mean arterial pressure, cerebral perfusion pressure, cerebral blood flow, and brain PO2. Administration of baicalin before the start of heat exposure significantly reduced the hyperthermia, intracranial hypertension, and the increased levels of NO2 -, glutamate, glycerol, lactate/pyruvate ratio, and DHBA in the hypothalamus that occurred during heatstroke. The heatstroke-induced increased levels of IL-1β and TNF-α in both the serum and hypothalamus, and renal and hepatic dysfunction were suppressed by baicalin pretreatment. In contrast, both the serum and hypothalamic levels of IL-10 were significantly elevated by baicalin during heatstroke. We successfully demonstrated that baicalin can be used as a prophylactic agent for heatstroke. In particular, baicalin may protect against cerebrovascular dysfunction and brain inflammation in heatstroke.

原文英語
頁(從 - 到)1024-1033
頁數10
期刊Neuropharmacology
52
發行號3
DOIs
出版狀態已發佈 - 三月 2007
對外發佈Yes

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ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Drug Discovery
  • Pharmacology

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