The expression profile and prognostic significance of metallothionein genes in colorectal cancer

Kuo Chen Hung, Tsui Chin Huang, Chia Hsiung Cheng, Ya Wen Cheng, Ding Yen Lin, Jhen Jia Fan, Kuen Haur Lee

研究成果: 雜誌貢獻文章

摘要

Colorectal cancer (CRC) is a heterogeneous disease resulting from the combined influence of many genetic factors. This complexity has caused the molecular characterization of CRC to remain uncharacterized, with a lack of clear gene markers associated with CRC and the prognosis of this disease. Thus, highly sensitive tumor markers for the detection of CRC are the most essential determinants of survival. In this study, we examined the simultaneous downregulation of the mRNA levels of six metallothionein (MT) genes in CRC cell lines and public CRC datasets for the first time. In addition, we detected downregulation of these six MT mRNAs’ levels in 30 pairs of tumor (T) and adjacent non-tumor (N) CRC specimens. In order to understand the potential prognostic relevance of these six MT genes and CRC, we presented a four-gene signature to evaluate the prognosis of CRC patients. Further discovery suggested that the four-gene signature (MT1F, MT1G, MT1L, and MT1X) predicted survival better than any combination of two-, three-, four-, five-, or six-gene models. In conclusion, this study is the first to report that simultaneous downregulation of six MT mRNAs’ levels in CRC patients, and their aberrant expression together, accurately predicted CRC patients’ outcomes.

原文英語
文章編號3849
期刊International Journal of Molecular Sciences
20
發行號16
DOIs
出版狀態已發佈 - 八月 2 2019

指紋

Metallothionein
genes
Colorectal Neoplasms
Genes
cancer
profiles
Messenger RNA
Down-Regulation
prognosis
Tumor Biomarkers
markers
Tumors
tumors
signatures
Cells
Survival
cultured cells
determinants
Cell Line

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

引用此文

The expression profile and prognostic significance of metallothionein genes in colorectal cancer. / Hung, Kuo Chen; Huang, Tsui Chin; Cheng, Chia Hsiung; Cheng, Ya Wen; Lin, Ding Yen; Fan, Jhen Jia; Lee, Kuen Haur.

於: International Journal of Molecular Sciences, 卷 20, 編號 16, 3849, 02.08.2019.

研究成果: 雜誌貢獻文章

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abstract = "Colorectal cancer (CRC) is a heterogeneous disease resulting from the combined influence of many genetic factors. This complexity has caused the molecular characterization of CRC to remain uncharacterized, with a lack of clear gene markers associated with CRC and the prognosis of this disease. Thus, highly sensitive tumor markers for the detection of CRC are the most essential determinants of survival. In this study, we examined the simultaneous downregulation of the mRNA levels of six metallothionein (MT) genes in CRC cell lines and public CRC datasets for the first time. In addition, we detected downregulation of these six MT mRNAs’ levels in 30 pairs of tumor (T) and adjacent non-tumor (N) CRC specimens. In order to understand the potential prognostic relevance of these six MT genes and CRC, we presented a four-gene signature to evaluate the prognosis of CRC patients. Further discovery suggested that the four-gene signature (MT1F, MT1G, MT1L, and MT1X) predicted survival better than any combination of two-, three-, four-, five-, or six-gene models. In conclusion, this study is the first to report that simultaneous downregulation of six MT mRNAs’ levels in CRC patients, and their aberrant expression together, accurately predicted CRC patients’ outcomes.",
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AU - Fan, Jhen Jia

AU - Lee, Kuen Haur

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