The elevation of l-3-hydroxybutyrate concentrations in the serum of aristolochic acid-treated mice

Chien Ming Chen; Tzu Chuan Huang; Jen Ai Lee

The elevation of l-3-hydroxybutyrate concentrations in the serum of aristolochic acid-treated mice

Chien Ming Chen, Tzu Chuan Huang, Jen Ai Lee

研究成果: 雜誌貢獻 › 文章 › 同行評審

摘要

The concentrations of L-3-hydroxybutyrate (L-3HB) in the serum of aristolochic acid (AA)-treated mice were determined by column-switching high-performance liquid chromatography (HPLC). The samples were derivatized with 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ) and determined fluorimetrically. Total 3-HB derivatives in serum were separated by an ODS column and then introduced to a sample loop for chiral separation by two tandem CHIRALCEL OD-RH columns. The results showed that the concentration of L-3HB in the serum of normal mice was 0.81 ± 0.15 μM compared to that of AA-treated mice with 4.33 ± 2.81 μM after 3 days and 8.65 ± 2.60 μM after 5 days of AA administration (p

原文	英語
頁（從 - 到）	25-30
頁數	6
期刊	International Journal of Bio-Science and Bio-Technology
卷	2
發行號	1
出版狀態	已發佈 - 三月 2010

ASJC Scopus subject areas

生物醫學工程
生物技術
生物工程
人工智慧

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The elevation of l-3-hydroxybutyrate concentrations in the serum of aristolochic acid-treated mice

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