摘要
The concentrations of L-3-hydroxybutyrate (L-3HB) in the serum of aristolochic acid (AA)-treated mice were determined by column-switching high-performance liquid chromatography (HPLC). The samples were derivatized with 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ) and determined fluorimetrically. Total 3-HB derivatives in serum were separated by an ODS column and then introduced to a sample loop for chiral separation by two tandem CHIRALCEL OD-RH columns. The results showed that the concentration of L-3HB in the serum of normal mice was 0.81 ± 0.15 μM compared to that of AA-treated mice with 4.33 ± 2.81 μM after 3 days and 8.65 ± 2.60 μM after 5 days of AA administration (p
原文 | 英語 |
---|---|
頁(從 - 到) | 25-30 |
頁數 | 6 |
期刊 | International Journal of Bio-Science and Bio-Technology |
卷 | 2 |
發行號 | 1 |
出版狀態 | 已發佈 - 3月 2010 |
ASJC Scopus subject areas
- 生物醫學工程
- 生物技術
- 生物工程
- 人工智慧