The elevation of l-3-hydroxybutyrate concentrations in the serum of aristolochic acid-treated mice

Chien Ming Chen; Tzu Chuan Huang; Jen Ai Lee

The elevation of l-3-hydroxybutyrate concentrations in the serum of aristolochic acid-treated mice

Chien Ming Chen, Tzu Chuan Huang, Jen Ai Lee

研究成果: 雜誌貢獻 › 文章 › 同行評審

摘要

The concentrations of L-3-hydroxybutyrate (L-3HB) in the serum of aristolochic acid (AA)-treated mice were determined by column-switching high-performance liquid chromatography (HPLC). The samples were derivatized with 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ) and determined fluorimetrically. Total 3-HB derivatives in serum were separated by an ODS column and then introduced to a sample loop for chiral separation by two tandem CHIRALCEL OD-RH columns. The results showed that the concentration of L-3HB in the serum of normal mice was 0.81 ± 0.15 μM compared to that of AA-treated mice with 4.33 ± 2.81 μM after 3 days and 8.65 ± 2.60 μM after 5 days of AA administration (p

原文	英語
頁（從 - 到）	25-30
頁數	6
期刊	International Journal of Bio-Science and Bio-Technology
卷	2
發行號	1
出版狀態	已發佈 - 3月 2010

ASJC Scopus subject areas

生物醫學工程
生物技術
生物工程
人工智慧

其它文件與鏈接

引用此

@article{123bf5ca3fab4aca8368798efcfa881f,

title = "The elevation of l-3-hydroxybutyrate concentrations in the serum of aristolochic acid-treated mice",

abstract = "The concentrations of L-3-hydroxybutyrate (L-3HB) in the serum of aristolochic acid (AA)-treated mice were determined by column-switching high-performance liquid chromatography (HPLC). The samples were derivatized with 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ) and determined fluorimetrically. Total 3-HB derivatives in serum were separated by an ODS column and then introduced to a sample loop for chiral separation by two tandem CHIRALCEL OD-RH columns. The results showed that the concentration of L-3HB in the serum of normal mice was 0.81 ± 0.15 μM compared to that of AA-treated mice with 4.33 ± 2.81 μM after 3 days and 8.65 ± 2.60 μM after 5 days of AA administration (p",

keywords = "Column-switching HPLC, L-3-hydroxybutyrate, Renal damage",

author = "Chen, {Chien Ming} and Huang, {Tzu Chuan} and Lee, {Jen Ai}",

year = "2010",

month = mar,

language = "English",

volume = "2",

pages = "25--30",

journal = "International Journal of Bio-Science and Bio-Technology",

issn = "2233-7849",

publisher = "Science and Engineering Research Support Society",

number = "1",

}

TY - JOUR

T1 - The elevation of l-3-hydroxybutyrate concentrations in the serum of aristolochic acid-treated mice

AU - Chen, Chien Ming

AU - Huang, Tzu Chuan

AU - Lee, Jen Ai

PY - 2010/3

Y1 - 2010/3

N2 - The concentrations of L-3-hydroxybutyrate (L-3HB) in the serum of aristolochic acid (AA)-treated mice were determined by column-switching high-performance liquid chromatography (HPLC). The samples were derivatized with 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ) and determined fluorimetrically. Total 3-HB derivatives in serum were separated by an ODS column and then introduced to a sample loop for chiral separation by two tandem CHIRALCEL OD-RH columns. The results showed that the concentration of L-3HB in the serum of normal mice was 0.81 ± 0.15 μM compared to that of AA-treated mice with 4.33 ± 2.81 μM after 3 days and 8.65 ± 2.60 μM after 5 days of AA administration (p

AB - The concentrations of L-3-hydroxybutyrate (L-3HB) in the serum of aristolochic acid (AA)-treated mice were determined by column-switching high-performance liquid chromatography (HPLC). The samples were derivatized with 4-nitro-7-piperazino-2,1,3-benzoxadiazole (NBD-PZ) and determined fluorimetrically. Total 3-HB derivatives in serum were separated by an ODS column and then introduced to a sample loop for chiral separation by two tandem CHIRALCEL OD-RH columns. The results showed that the concentration of L-3HB in the serum of normal mice was 0.81 ± 0.15 μM compared to that of AA-treated mice with 4.33 ± 2.81 μM after 3 days and 8.65 ± 2.60 μM after 5 days of AA administration (p

KW - Column-switching HPLC

KW - L-3-hydroxybutyrate

KW - Renal damage

UR - http://www.scopus.com/inward/record.url?scp=79960738132&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79960738132&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:79960738132

VL - 2

SP - 25

EP - 30

JO - International Journal of Bio-Science and Bio-Technology

JF - International Journal of Bio-Science and Bio-Technology

SN - 2233-7849

IS - 1

ER -

The elevation of l-3-hydroxybutyrate concentrations in the serum of aristolochic acid-treated mice

摘要

ASJC Scopus subject areas

其它文件與鏈接

指紋

引用此