The EGF/hnRNP Q1 axis is involved in tumorigenesis via the regulation of cell cycle-related genes

Yu Chu Wang, Kung Chao Chang, Bo Wen Lin, Jenq Chang Lee, Chien Hsien Lai, Li Jyuan Lin, Yun Yen, Chang Shen Lin, Shiang Jie Yang, Peng Chan Lin, Chung Ta Lee, Liang Yi Hung

研究成果: 雜誌貢獻文章

摘要

Heterogeneous nuclear ribonucleoprotein (hnRNP) Q1, an RNA-binding protein, has been implicated in many post-transcriptional processes, including RNA metabolism and mRNA splicing and translation. However, the role of hnRNP Q1 in tumorigenesis remains unclear. We previously performed RNA immunoprecipitation (RIP)-seq analysis to identify hnRNP Q1-interacting mRNAs and found that hnRNP Q1 targets a group of genes that are involved in mitotic regulation, including Aurora-A. Here, we demonstrate that altering the hnRNP Q1 level influences the expression of the Aurora-A protein, but not its mRNA. Stimulation with epidermal growth factor (EGF) enhances both binding between hnRNP Q1 and Aurora-A mRNA as well as the efficacy of the hnRNP Q1-induced translation of Aurora-A mRNA. The EGF/hnRNP Q1-induced translation of Aurora-A mRNA is mediated by the mTOR and ERK pathways. In addition, we show that hnRNP Q1 up-regulates the translation of a group of spindle assembly checkpoint (SAC) genes. hnRNP Q1 overexpression is positively correlated with the levels of Aurora-A and the SAC genes in human colorectal cancer tissues. In summary, our data suggest that hnRNP Q1 plays an important role in regulating the expression of a group of cell cycle-related genes. Therefore, it may contribute to tumorigenesis by up-regulating the translation of these genes in colorectal cancer.

原文英語
文章編號70
頁(從 - 到)70
期刊Experimental and Molecular Medicine
50
發行號6
DOIs
出版狀態已發佈 - 六月 1 2018

指紋

Heterogeneous-Nuclear Ribonucleoproteins
cdc Genes
Epidermal Growth Factor
Carcinogenesis
Genes
Cells
Messenger RNA
M Phase Cell Cycle Checkpoints
Colorectal Neoplasms
RNA
RNA-Binding Proteins
MAP Kinase Signaling System
Protein Biosynthesis
Immunoprecipitation
Metabolism
Up-Regulation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

引用此文

Wang, Y. C., Chang, K. C., Lin, B. W., Lee, J. C., Lai, C. H., Lin, L. J., ... Hung, L. Y. (2018). The EGF/hnRNP Q1 axis is involved in tumorigenesis via the regulation of cell cycle-related genes. Experimental and Molecular Medicine, 50(6), 70. [70]. https://doi.org/10.1038/s12276-018-0101-6

The EGF/hnRNP Q1 axis is involved in tumorigenesis via the regulation of cell cycle-related genes. / Wang, Yu Chu; Chang, Kung Chao; Lin, Bo Wen; Lee, Jenq Chang; Lai, Chien Hsien; Lin, Li Jyuan; Yen, Yun; Lin, Chang Shen; Yang, Shiang Jie; Lin, Peng Chan; Lee, Chung Ta; Hung, Liang Yi.

於: Experimental and Molecular Medicine, 卷 50, 編號 6, 70, 01.06.2018, p. 70.

研究成果: 雜誌貢獻文章

Wang, YC, Chang, KC, Lin, BW, Lee, JC, Lai, CH, Lin, LJ, Yen, Y, Lin, CS, Yang, SJ, Lin, PC, Lee, CT & Hung, LY 2018, 'The EGF/hnRNP Q1 axis is involved in tumorigenesis via the regulation of cell cycle-related genes', Experimental and Molecular Medicine, 卷 50, 編號 6, 70, 頁 70. https://doi.org/10.1038/s12276-018-0101-6
Wang, Yu Chu ; Chang, Kung Chao ; Lin, Bo Wen ; Lee, Jenq Chang ; Lai, Chien Hsien ; Lin, Li Jyuan ; Yen, Yun ; Lin, Chang Shen ; Yang, Shiang Jie ; Lin, Peng Chan ; Lee, Chung Ta ; Hung, Liang Yi. / The EGF/hnRNP Q1 axis is involved in tumorigenesis via the regulation of cell cycle-related genes. 於: Experimental and Molecular Medicine. 2018 ; 卷 50, 編號 6. 頁 70.
@article{5fec8c0f04da48b49ed965099aec818b,
title = "The EGF/hnRNP Q1 axis is involved in tumorigenesis via the regulation of cell cycle-related genes",
abstract = "Heterogeneous nuclear ribonucleoprotein (hnRNP) Q1, an RNA-binding protein, has been implicated in many post-transcriptional processes, including RNA metabolism and mRNA splicing and translation. However, the role of hnRNP Q1 in tumorigenesis remains unclear. We previously performed RNA immunoprecipitation (RIP)-seq analysis to identify hnRNP Q1-interacting mRNAs and found that hnRNP Q1 targets a group of genes that are involved in mitotic regulation, including Aurora-A. Here, we demonstrate that altering the hnRNP Q1 level influences the expression of the Aurora-A protein, but not its mRNA. Stimulation with epidermal growth factor (EGF) enhances both binding between hnRNP Q1 and Aurora-A mRNA as well as the efficacy of the hnRNP Q1-induced translation of Aurora-A mRNA. The EGF/hnRNP Q1-induced translation of Aurora-A mRNA is mediated by the mTOR and ERK pathways. In addition, we show that hnRNP Q1 up-regulates the translation of a group of spindle assembly checkpoint (SAC) genes. hnRNP Q1 overexpression is positively correlated with the levels of Aurora-A and the SAC genes in human colorectal cancer tissues. In summary, our data suggest that hnRNP Q1 plays an important role in regulating the expression of a group of cell cycle-related genes. Therefore, it may contribute to tumorigenesis by up-regulating the translation of these genes in colorectal cancer.",
author = "Wang, {Yu Chu} and Chang, {Kung Chao} and Lin, {Bo Wen} and Lee, {Jenq Chang} and Lai, {Chien Hsien} and Lin, {Li Jyuan} and Yun Yen and Lin, {Chang Shen} and Yang, {Shiang Jie} and Lin, {Peng Chan} and Lee, {Chung Ta} and Hung, {Liang Yi}",
year = "2018",
month = "6",
day = "1",
doi = "10.1038/s12276-018-0101-6",
language = "English",
volume = "50",
pages = "70",
journal = "Experimental and Molecular Medicine",
issn = "1226-3613",
publisher = "Korean Society of Med. Biochemistry and Mol. Biology",
number = "6",

}

TY - JOUR

T1 - The EGF/hnRNP Q1 axis is involved in tumorigenesis via the regulation of cell cycle-related genes

AU - Wang, Yu Chu

AU - Chang, Kung Chao

AU - Lin, Bo Wen

AU - Lee, Jenq Chang

AU - Lai, Chien Hsien

AU - Lin, Li Jyuan

AU - Yen, Yun

AU - Lin, Chang Shen

AU - Yang, Shiang Jie

AU - Lin, Peng Chan

AU - Lee, Chung Ta

AU - Hung, Liang Yi

PY - 2018/6/1

Y1 - 2018/6/1

N2 - Heterogeneous nuclear ribonucleoprotein (hnRNP) Q1, an RNA-binding protein, has been implicated in many post-transcriptional processes, including RNA metabolism and mRNA splicing and translation. However, the role of hnRNP Q1 in tumorigenesis remains unclear. We previously performed RNA immunoprecipitation (RIP)-seq analysis to identify hnRNP Q1-interacting mRNAs and found that hnRNP Q1 targets a group of genes that are involved in mitotic regulation, including Aurora-A. Here, we demonstrate that altering the hnRNP Q1 level influences the expression of the Aurora-A protein, but not its mRNA. Stimulation with epidermal growth factor (EGF) enhances both binding between hnRNP Q1 and Aurora-A mRNA as well as the efficacy of the hnRNP Q1-induced translation of Aurora-A mRNA. The EGF/hnRNP Q1-induced translation of Aurora-A mRNA is mediated by the mTOR and ERK pathways. In addition, we show that hnRNP Q1 up-regulates the translation of a group of spindle assembly checkpoint (SAC) genes. hnRNP Q1 overexpression is positively correlated with the levels of Aurora-A and the SAC genes in human colorectal cancer tissues. In summary, our data suggest that hnRNP Q1 plays an important role in regulating the expression of a group of cell cycle-related genes. Therefore, it may contribute to tumorigenesis by up-regulating the translation of these genes in colorectal cancer.

AB - Heterogeneous nuclear ribonucleoprotein (hnRNP) Q1, an RNA-binding protein, has been implicated in many post-transcriptional processes, including RNA metabolism and mRNA splicing and translation. However, the role of hnRNP Q1 in tumorigenesis remains unclear. We previously performed RNA immunoprecipitation (RIP)-seq analysis to identify hnRNP Q1-interacting mRNAs and found that hnRNP Q1 targets a group of genes that are involved in mitotic regulation, including Aurora-A. Here, we demonstrate that altering the hnRNP Q1 level influences the expression of the Aurora-A protein, but not its mRNA. Stimulation with epidermal growth factor (EGF) enhances both binding between hnRNP Q1 and Aurora-A mRNA as well as the efficacy of the hnRNP Q1-induced translation of Aurora-A mRNA. The EGF/hnRNP Q1-induced translation of Aurora-A mRNA is mediated by the mTOR and ERK pathways. In addition, we show that hnRNP Q1 up-regulates the translation of a group of spindle assembly checkpoint (SAC) genes. hnRNP Q1 overexpression is positively correlated with the levels of Aurora-A and the SAC genes in human colorectal cancer tissues. In summary, our data suggest that hnRNP Q1 plays an important role in regulating the expression of a group of cell cycle-related genes. Therefore, it may contribute to tumorigenesis by up-regulating the translation of these genes in colorectal cancer.

UR - http://www.scopus.com/inward/record.url?scp=85048291062&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85048291062&partnerID=8YFLogxK

U2 - 10.1038/s12276-018-0101-6

DO - 10.1038/s12276-018-0101-6

M3 - Article

VL - 50

SP - 70

JO - Experimental and Molecular Medicine

JF - Experimental and Molecular Medicine

SN - 1226-3613

IS - 6

M1 - 70

ER -