The effects of negative pressure treatment on the extracellular matrix gene expression and protein production of fibroblasts

Pei Yu Chen, Chih Chin Hsu, Kai Chiang Yang, Chang Chin Wu, Chung Li Wang

研究成果: 雜誌貢獻文章

4 引文 斯高帕斯(Scopus)

摘要

Although negative pressure wound therapy (NPWT) has been suggested for the management of acute and chronic wounds, the underlying mechanism of NPWT on the cellular level is not fully understood. Using a primary fibroblast culture model with controlled negative pressure, the cellular responses to the negative pressure was investigated. The present results showed that cell proliferation of fibroblasts was not affected under negative pressure. The mRNA expressions of collagen type I, collagen type III, and fibronectin were down-regulated when cells treated with negative pressure for once only. However, those mRNA expressions were up-regulated when cells subjected to periodical negative pressure consecutively for 5 days. The protein production of TGF-β1 was enhanced, while the TIMP-1, decorin, and IL-6 were not influenced. The pro-MMP-9 was not stimulated when fibroblasts subjected to negative pressure. The regulations of extracellular matrix production in fibroblasts provides an insight into the cellular mechanisms of NPWT.
原文英語
文章編號10458
頁(從 - 到)1662-1668
頁數7
期刊Process Biochemistry
50
發行號10
DOIs
出版狀態已發佈 - 十月 1 2015

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering

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