The effects of microenvironment on wound healing by keratinocytes derived from mesenchymal stem cells

Yi-Han Lin, Keng-Yen Fu, Po-Da Hong, Hsu Ma, Nien-Hsien Liou, Kuo-Hsing Ma, Jiang-Chuan Liu, Kun-Lun Huang, Lien-Guo Dai, Shun-Cheng Chang, Yi-Hsin Chan, Shyi-Gen Chen, Tim-Mo Chen, Niann-Tzyy Dai

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5 引文 斯高帕斯(Scopus)

摘要

ABSTRACT: Embryonic stem cells (ESCs) are pluripotent cells that can differentiate into various cell types, including keratinocyte-like cells, within suitable microniches. In this study, we aimed to investigate the effects of culture media, cell coculture, and a tissue-engineering biocomposite on the differentiation of mouse ESCs (MESCs) into keratinocyte-like cells and applied these cells to a surgical skin wound model. MESCs from BALB/c mice (ESC26GJ), which were transfected using pCX-EGFP expressing green fluorescence, were used to track MESC-derived keratinocytes. Weak expression of the keratinocyte early marker Cytokeratin 14 (CK-14) was observed up to 12 days when MESCs were cultured in a keratinocyte culture medium on tissue culture plastic and on a gelatin/collagen/polycaprolactone (GCP) biocomposite. MESCs cocultured with human keratinocyte cells (HKCs) also expressed CK-14, but did not express CK-14 when cocultured with human fibroblast cells (HFCs). Furthermore, CK-14 expression was observed when MESCs were cocultured by seeding HKCs or HFCs on the same or opposite side of the GCP biocomposite. The highest CK-14 expression was observed by seeding MESCs and HKCs on the same side of the GCP composite and with HFCs on the opposite side. To verify the effectiveness of wound healing in vivo, adipose-derived stem cells were applied to treat surgical wounds in nude mice. An obvious epidermis multilayer and better collagen deposition during wound healing were observed, as assessed by Masson staining. This study demonstrated the potential of keratinocyte-like differentiation from mesenchymal stem cells for use in promoting wound closure and skin regeneration. Copyright © 2013 Lippincott Williams & Wilkins.
原文英語
頁(從 - 到)S67-S74
期刊Annals of Plastic Surgery
71
發行號SUPPL.1
DOIs
出版狀態已發佈 - 2013
對外發佈Yes

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