The effect of gelatin-chondroitin sulfate-hyaluronic acid skin substitute on wound healing in SCID mice

Tzu Wei Wang, Jui Sheng Sun, Hsi Chin Wu, Yang Hwei Tsuang, Wen Hsi Wang, Feng Huei Lin

研究成果: 雜誌貢獻文章同行評審

102 引文 斯高帕斯(Scopus)

摘要

Tissue-engineered skin substitutes provided a feasibility to overcome the shortage of skin autograft by culturing keratinocytes and dermal fibroblasts in vitro. In this study, we applied bi-layer gelatin-chondrointin-6-sulfate-hyaluronic acid (gelatin-C6S-HA) biomatrices onto the severe combined immunodeficiency (SCID) mice to evaluate its effect on promoting wound healing. Human foreskin keratinocytes and dermal fibroblasts were cultured with reconstructed skin equivalent (rSE) for 7 days. The rSE was then grafted to the dorsum of SCID mice to evaluate its biocompatibility by histologic and immunohistochemistry analysis. The results showed that human epidermis were well-developed with the expression of differentiated markers and basement membrane-specific proteins at 4 weeks. After implantation, the percentages of skin graft take were satisfactory, while cell-seeded group was better than non-cell-seeded one. The basement membrane proteins including laminin, type IV collagen, type VII collagen, integrin α6, and integrin β4 were all detected at the dermal-epidermal junction, which showed a continuous structure in the 4 weeks after grafting. This bi-layer gelatin-C6S-HA skin substitute not only has positive effect on promoting wound healing, but also has high rate of graft take. This rSE would have the potential to be applied on the extensively and deeply burned patients who suffer from severe skin defect in the near future.

原文英語
頁(從 - 到)5689-5697
頁數9
期刊Biomaterials
27
發行號33
DOIs
出版狀態已發佈 - 11月 2006
對外發佈

ASJC Scopus subject areas

  • 生物物理學
  • 生物工程
  • 陶瓷和複合材料
  • 生物材料
  • 材料力學

指紋

深入研究「The effect of gelatin-chondroitin sulfate-hyaluronic acid skin substitute on wound healing in SCID mice」主題。共同形成了獨特的指紋。

引用此