The Effect of Different Harvest Strategies on the Nucleated Cell Yields of Bone Marrow Collection

Tso Fu Wang, Sung Chao Chu, Shu Huey Chen, Kuan Po Huang, Yu Chieh Su, Dian Kun Li, Ming Hwang Shyr, Chu Yu Chang, Hsi Hsiu Tsai, Ruey Ho Kao

研究成果: 雜誌貢獻文章

10 引文 (Scopus)

摘要

To improve bone marrow (BM) harvest of the volunteer donors in our institute, we changed from the single-hole needle to the multi-side-hole needle after March 2002, and examined the midway total nucleated cell (TNC) counts during collection after September 2004. The aims of this retrospective study were to evaluate BM harvest yields obtained through different strategies and to examine the correlation between final and midway BM harvests. The distribution of BM harvesting by different strategies was 235 donors with single-hole needles (group A), 389 donors with 5-side-hole needles (group B), and 365 donors with 5-side-hole needles and midway TNC counts (group C). The nucleated cell density of the collected BM was significantly improved by modifying the harvest strategy (0.202 × 108/mL in group A, 0.219 × 108/mL in group B, and 0.250 × 108/mL in group C; P <.001). The percentage of unacceptable TNC dose (8/kg) was also decreased in all 3 groups (to 5.9%, 3.6%, and 0%, respectively; P <.001). Multiple regression analysis revealed that donor weight, white blood cell count, and harvest strategy were positively correlated with BM TNC density (P <.001), whereas harvested BM volume was negatively correlated with TNC density (P <.001). On linear regression analysis, highly significant correlations were noted between midway and final TNC densities (r = 0.8774; P <.001) as well as between harvested BM volume and TNC count (r = 0.7937; P <.001). Changing the harvesting needle and checking the midway TNC count improved the harvest outcome.
原文英語
頁(從 - 到)351-355
頁數5
期刊Biology of Blood and Marrow Transplantation
17
發行號3
DOIs
出版狀態已發佈 - 三月 2011
對外發佈Yes

指紋

Bone Marrow Cells
Cell Count
Bone Marrow
Needles
Tissue Donors
Regression Analysis
Leukocyte Count
Volunteers
Linear Models
Retrospective Studies
Weights and Measures

ASJC Scopus subject areas

  • Transplantation
  • Hematology

引用此文

The Effect of Different Harvest Strategies on the Nucleated Cell Yields of Bone Marrow Collection. / Wang, Tso Fu; Chu, Sung Chao; Chen, Shu Huey; Huang, Kuan Po; Su, Yu Chieh; Li, Dian Kun; Shyr, Ming Hwang; Chang, Chu Yu; Tsai, Hsi Hsiu; Kao, Ruey Ho.

於: Biology of Blood and Marrow Transplantation, 卷 17, 編號 3, 03.2011, p. 351-355.

研究成果: 雜誌貢獻文章

Wang, Tso Fu ; Chu, Sung Chao ; Chen, Shu Huey ; Huang, Kuan Po ; Su, Yu Chieh ; Li, Dian Kun ; Shyr, Ming Hwang ; Chang, Chu Yu ; Tsai, Hsi Hsiu ; Kao, Ruey Ho. / The Effect of Different Harvest Strategies on the Nucleated Cell Yields of Bone Marrow Collection. 於: Biology of Blood and Marrow Transplantation. 2011 ; 卷 17, 編號 3. 頁 351-355.
@article{f2aef52e7fac4a2db699b71b53428b83,
title = "The Effect of Different Harvest Strategies on the Nucleated Cell Yields of Bone Marrow Collection",
abstract = "To improve bone marrow (BM) harvest of the volunteer donors in our institute, we changed from the single-hole needle to the multi-side-hole needle after March 2002, and examined the midway total nucleated cell (TNC) counts during collection after September 2004. The aims of this retrospective study were to evaluate BM harvest yields obtained through different strategies and to examine the correlation between final and midway BM harvests. The distribution of BM harvesting by different strategies was 235 donors with single-hole needles (group A), 389 donors with 5-side-hole needles (group B), and 365 donors with 5-side-hole needles and midway TNC counts (group C). The nucleated cell density of the collected BM was significantly improved by modifying the harvest strategy (0.202 × 108/mL in group A, 0.219 × 108/mL in group B, and 0.250 × 108/mL in group C; P <.001). The percentage of unacceptable TNC dose (8/kg) was also decreased in all 3 groups (to 5.9{\%}, 3.6{\%}, and 0{\%}, respectively; P <.001). Multiple regression analysis revealed that donor weight, white blood cell count, and harvest strategy were positively correlated with BM TNC density (P <.001), whereas harvested BM volume was negatively correlated with TNC density (P <.001). On linear regression analysis, highly significant correlations were noted between midway and final TNC densities (r = 0.8774; P <.001) as well as between harvested BM volume and TNC count (r = 0.7937; P <.001). Changing the harvesting needle and checking the midway TNC count improved the harvest outcome.",
keywords = "Healthy donor, Hematopoietic stem cell, Total nucleated cell, Transplantation",
author = "Wang, {Tso Fu} and Chu, {Sung Chao} and Chen, {Shu Huey} and Huang, {Kuan Po} and Su, {Yu Chieh} and Li, {Dian Kun} and Shyr, {Ming Hwang} and Chang, {Chu Yu} and Tsai, {Hsi Hsiu} and Kao, {Ruey Ho}",
year = "2011",
month = "3",
doi = "10.1016/j.bbmt.2010.05.013",
language = "English",
volume = "17",
pages = "351--355",
journal = "Biology of Blood and Marrow Transplantation",
issn = "1083-8791",
publisher = "Elsevier Inc.",
number = "3",

}

TY - JOUR

T1 - The Effect of Different Harvest Strategies on the Nucleated Cell Yields of Bone Marrow Collection

AU - Wang, Tso Fu

AU - Chu, Sung Chao

AU - Chen, Shu Huey

AU - Huang, Kuan Po

AU - Su, Yu Chieh

AU - Li, Dian Kun

AU - Shyr, Ming Hwang

AU - Chang, Chu Yu

AU - Tsai, Hsi Hsiu

AU - Kao, Ruey Ho

PY - 2011/3

Y1 - 2011/3

N2 - To improve bone marrow (BM) harvest of the volunteer donors in our institute, we changed from the single-hole needle to the multi-side-hole needle after March 2002, and examined the midway total nucleated cell (TNC) counts during collection after September 2004. The aims of this retrospective study were to evaluate BM harvest yields obtained through different strategies and to examine the correlation between final and midway BM harvests. The distribution of BM harvesting by different strategies was 235 donors with single-hole needles (group A), 389 donors with 5-side-hole needles (group B), and 365 donors with 5-side-hole needles and midway TNC counts (group C). The nucleated cell density of the collected BM was significantly improved by modifying the harvest strategy (0.202 × 108/mL in group A, 0.219 × 108/mL in group B, and 0.250 × 108/mL in group C; P <.001). The percentage of unacceptable TNC dose (8/kg) was also decreased in all 3 groups (to 5.9%, 3.6%, and 0%, respectively; P <.001). Multiple regression analysis revealed that donor weight, white blood cell count, and harvest strategy were positively correlated with BM TNC density (P <.001), whereas harvested BM volume was negatively correlated with TNC density (P <.001). On linear regression analysis, highly significant correlations were noted between midway and final TNC densities (r = 0.8774; P <.001) as well as between harvested BM volume and TNC count (r = 0.7937; P <.001). Changing the harvesting needle and checking the midway TNC count improved the harvest outcome.

AB - To improve bone marrow (BM) harvest of the volunteer donors in our institute, we changed from the single-hole needle to the multi-side-hole needle after March 2002, and examined the midway total nucleated cell (TNC) counts during collection after September 2004. The aims of this retrospective study were to evaluate BM harvest yields obtained through different strategies and to examine the correlation between final and midway BM harvests. The distribution of BM harvesting by different strategies was 235 donors with single-hole needles (group A), 389 donors with 5-side-hole needles (group B), and 365 donors with 5-side-hole needles and midway TNC counts (group C). The nucleated cell density of the collected BM was significantly improved by modifying the harvest strategy (0.202 × 108/mL in group A, 0.219 × 108/mL in group B, and 0.250 × 108/mL in group C; P <.001). The percentage of unacceptable TNC dose (8/kg) was also decreased in all 3 groups (to 5.9%, 3.6%, and 0%, respectively; P <.001). Multiple regression analysis revealed that donor weight, white blood cell count, and harvest strategy were positively correlated with BM TNC density (P <.001), whereas harvested BM volume was negatively correlated with TNC density (P <.001). On linear regression analysis, highly significant correlations were noted between midway and final TNC densities (r = 0.8774; P <.001) as well as between harvested BM volume and TNC count (r = 0.7937; P <.001). Changing the harvesting needle and checking the midway TNC count improved the harvest outcome.

KW - Healthy donor

KW - Hematopoietic stem cell

KW - Total nucleated cell

KW - Transplantation

UR - http://www.scopus.com/inward/record.url?scp=79551689551&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79551689551&partnerID=8YFLogxK

U2 - 10.1016/j.bbmt.2010.05.013

DO - 10.1016/j.bbmt.2010.05.013

M3 - Article

C2 - 20553925

AN - SCOPUS:79551689551

VL - 17

SP - 351

EP - 355

JO - Biology of Blood and Marrow Transplantation

JF - Biology of Blood and Marrow Transplantation

SN - 1083-8791

IS - 3

ER -