The effect of Chinese herbal medicines on TNF-α induced matrix metalloproteinase-1, -9 activities and interleukin-8 secretion

Mei Hsien Lee, Yi Yuan Yang, Yu-Hui Tsai, Yueh Lun Lee, Po Yuan Huang, I. Jen Huang, Kur Ta Cheng, Sy Jye Leu

研究成果: 雜誌貢獻文章

5 引文 (Scopus)

摘要

Matrix metalloproteinases (MMPs) play an important role in normal physiological functions and pathological processes. They are involved in normal skin functions as well as in the aging, healing, embryonic development, reproduction, and inflammatory responses of skin. Previous studies report that both high MMP-1 and MMP-2 activities were found in the skin of patients with dermatitis, and large amounts of MMP-9 have been reported to be accumulated in unhealed wounds. Interleukin-8 (IL-8), a C-X-C chemokine, may mediate neutrophil recruitment and activation and is involved in various inflammatory skin diseases. In this study, eleven Chinese herbal medicines were analyzed for their potential as anti-inflammatory agents using human fibroblast WS-1 cell lines. The results indicate MMP-1 and -9, but not MMP-2, were induced by TNF-α treatment in WS-1 cells. However, when WS-1 cells were pre-treated with eleven Chinese herbal medicines before TNF-α stimulation, all these herbal medicines suppressed TNF-α-stimulated MMP-1 activity in WS-1 cells as analyzed by casein zymography. In addition, the suppression of MMP-9 activity was also observed when WS-1 cells were treated with either Paeonia suffruticosa, Scutellaria baicalensis, Saposhnikovia divaricata, Dioscorea opposita, Rubus chingii, or Salvia miltiorrhiza. Of which, R. chingii significantly inhibited IL-8 secretion induced by TNF-α treatment as well. These results revealed that some novel components present in these Chinese herbal medicines may be used for the treatment of inflammatory responses in skin cells.
原文英語
頁(從 - 到)301-309
頁數9
期刊Botanical Studies
49
發行號4
出版狀態已發佈 - 十月 2008

指紋

interstitial collagenase
herbal medicines
interleukin-8
skin (animal)
secretion
gelatinase A
gelatinase B
cells
inflammation
Dioscorea oppositifolia
Paeonia suffruticosa
Scutellaria baicalensis
Salvia miltiorrhiza
anti-inflammatory agents
skin diseases
metalloproteinases
dermatitis
animal injuries
fibroblasts
neutrophils

ASJC Scopus subject areas

  • Plant Science

引用此文

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title = "The effect of Chinese herbal medicines on TNF-α induced matrix metalloproteinase-1, -9 activities and interleukin-8 secretion",
abstract = "Matrix metalloproteinases (MMPs) play an important role in normal physiological functions and pathological processes. They are involved in normal skin functions as well as in the aging, healing, embryonic development, reproduction, and inflammatory responses of skin. Previous studies report that both high MMP-1 and MMP-2 activities were found in the skin of patients with dermatitis, and large amounts of MMP-9 have been reported to be accumulated in unhealed wounds. Interleukin-8 (IL-8), a C-X-C chemokine, may mediate neutrophil recruitment and activation and is involved in various inflammatory skin diseases. In this study, eleven Chinese herbal medicines were analyzed for their potential as anti-inflammatory agents using human fibroblast WS-1 cell lines. The results indicate MMP-1 and -9, but not MMP-2, were induced by TNF-α treatment in WS-1 cells. However, when WS-1 cells were pre-treated with eleven Chinese herbal medicines before TNF-α stimulation, all these herbal medicines suppressed TNF-α-stimulated MMP-1 activity in WS-1 cells as analyzed by casein zymography. In addition, the suppression of MMP-9 activity was also observed when WS-1 cells were treated with either Paeonia suffruticosa, Scutellaria baicalensis, Saposhnikovia divaricata, Dioscorea opposita, Rubus chingii, or Salvia miltiorrhiza. Of which, R. chingii significantly inhibited IL-8 secretion induced by TNF-α treatment as well. These results revealed that some novel components present in these Chinese herbal medicines may be used for the treatment of inflammatory responses in skin cells.",
keywords = "Chinese herbal medicines, IL-8, MMP-1,-2,-9",
author = "Lee, {Mei Hsien} and Yang, {Yi Yuan} and Yu-Hui Tsai and Lee, {Yueh Lun} and Huang, {Po Yuan} and Huang, {I. Jen} and Cheng, {Kur Ta} and Leu, {Sy Jye}",
year = "2008",
month = "10",
language = "English",
volume = "49",
pages = "301--309",
journal = "Botanical Studies",
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T1 - The effect of Chinese herbal medicines on TNF-α induced matrix metalloproteinase-1, -9 activities and interleukin-8 secretion

AU - Lee, Mei Hsien

AU - Yang, Yi Yuan

AU - Tsai, Yu-Hui

AU - Lee, Yueh Lun

AU - Huang, Po Yuan

AU - Huang, I. Jen

AU - Cheng, Kur Ta

AU - Leu, Sy Jye

PY - 2008/10

Y1 - 2008/10

N2 - Matrix metalloproteinases (MMPs) play an important role in normal physiological functions and pathological processes. They are involved in normal skin functions as well as in the aging, healing, embryonic development, reproduction, and inflammatory responses of skin. Previous studies report that both high MMP-1 and MMP-2 activities were found in the skin of patients with dermatitis, and large amounts of MMP-9 have been reported to be accumulated in unhealed wounds. Interleukin-8 (IL-8), a C-X-C chemokine, may mediate neutrophil recruitment and activation and is involved in various inflammatory skin diseases. In this study, eleven Chinese herbal medicines were analyzed for their potential as anti-inflammatory agents using human fibroblast WS-1 cell lines. The results indicate MMP-1 and -9, but not MMP-2, were induced by TNF-α treatment in WS-1 cells. However, when WS-1 cells were pre-treated with eleven Chinese herbal medicines before TNF-α stimulation, all these herbal medicines suppressed TNF-α-stimulated MMP-1 activity in WS-1 cells as analyzed by casein zymography. In addition, the suppression of MMP-9 activity was also observed when WS-1 cells were treated with either Paeonia suffruticosa, Scutellaria baicalensis, Saposhnikovia divaricata, Dioscorea opposita, Rubus chingii, or Salvia miltiorrhiza. Of which, R. chingii significantly inhibited IL-8 secretion induced by TNF-α treatment as well. These results revealed that some novel components present in these Chinese herbal medicines may be used for the treatment of inflammatory responses in skin cells.

AB - Matrix metalloproteinases (MMPs) play an important role in normal physiological functions and pathological processes. They are involved in normal skin functions as well as in the aging, healing, embryonic development, reproduction, and inflammatory responses of skin. Previous studies report that both high MMP-1 and MMP-2 activities were found in the skin of patients with dermatitis, and large amounts of MMP-9 have been reported to be accumulated in unhealed wounds. Interleukin-8 (IL-8), a C-X-C chemokine, may mediate neutrophil recruitment and activation and is involved in various inflammatory skin diseases. In this study, eleven Chinese herbal medicines were analyzed for their potential as anti-inflammatory agents using human fibroblast WS-1 cell lines. The results indicate MMP-1 and -9, but not MMP-2, were induced by TNF-α treatment in WS-1 cells. However, when WS-1 cells were pre-treated with eleven Chinese herbal medicines before TNF-α stimulation, all these herbal medicines suppressed TNF-α-stimulated MMP-1 activity in WS-1 cells as analyzed by casein zymography. In addition, the suppression of MMP-9 activity was also observed when WS-1 cells were treated with either Paeonia suffruticosa, Scutellaria baicalensis, Saposhnikovia divaricata, Dioscorea opposita, Rubus chingii, or Salvia miltiorrhiza. Of which, R. chingii significantly inhibited IL-8 secretion induced by TNF-α treatment as well. These results revealed that some novel components present in these Chinese herbal medicines may be used for the treatment of inflammatory responses in skin cells.

KW - Chinese herbal medicines

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