The deduced probable human leukocyte antigen haplotype associatedwith human leukocyte antigen low incidence allele B*40

36 (A*02-B*40:36-DRB1*12) in Taiwanese unrelated hematopoietic bone marrow stem cell donors

Kuo Liang Yang, Reuy Ho Kao, Chin Lon Lin, Py Yu Lin

研究成果: 雜誌貢獻文章

摘要

Objective: Human leukocyte antigen (HLA)-B*40:36 is a low incidence allele in the HLA-B locus. This study reports the ethnicity of B*40:36 and the deduced probable HLA haplotype associated with HLA-B*40:36 in Taiwanese unrelated bone marrow hematopoietic stem cell donors. Materials and methods: A sequence-based typing method was employed to confirm that the low incidence allele was present. Polymerase chain reaction was performed to amplify exons 2 and 3 in the HLA-A and HLA-B loci and exon 2 in the HLA-DRB1 locus using group-specific primer sets. The amplicons were sequenced using the BigDye Terminator Cycle Sequencing Ready Reaction Kit in both directions. Results: The DNA sequence of B*40:36 is identical to B*40:01:01 in exons 2 and 3, except for a one nucleotide substitution at residue 419 (A→T), which results in a one amino acid replacement at position 116 (Y→F; tyrosine→phenylalanine). We deduced the probable HLA haplotype in an association with B*40:36 in Taiwanese to be A*02-B*40:36-DRB1*12. Conclusion: Information on the deduced probable HLA haplotype in an association with the low incidence B*40:36 allele that we report here is of value for HLA testing laboratories for reference purposes. In addition, it can be used by stem cell transplantation donor search coordinators to determine the likelihood of finding compatible donors in unrelated bone marrow donor registries when a patient has this uncommon HLA allele.
原文英語
頁(從 - 到)157-160
頁數4
期刊Tzu Chi Medical Journal
26
發行號4
DOIs
出版狀態已發佈 - 一月 1 2014
對外發佈Yes

指紋

HLA Antigens
Bone Marrow Cells
Haplotypes
Stem Cells
Alleles
Tissue Donors
Incidence
Exons
Bone Marrow
Unrelated Donors
Stem Cell Transplantation
Hematopoietic Stem Cells
Registries
Nucleotides
Amino Acids
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Medicine(all)

引用此文

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title = "The deduced probable human leukocyte antigen haplotype associatedwith human leukocyte antigen low incidence allele B*40: 36 (A*02-B*40:36-DRB1*12) in Taiwanese unrelated hematopoietic bone marrow stem cell donors",
abstract = "Objective: Human leukocyte antigen (HLA)-B*40:36 is a low incidence allele in the HLA-B locus. This study reports the ethnicity of B*40:36 and the deduced probable HLA haplotype associated with HLA-B*40:36 in Taiwanese unrelated bone marrow hematopoietic stem cell donors. Materials and methods: A sequence-based typing method was employed to confirm that the low incidence allele was present. Polymerase chain reaction was performed to amplify exons 2 and 3 in the HLA-A and HLA-B loci and exon 2 in the HLA-DRB1 locus using group-specific primer sets. The amplicons were sequenced using the BigDye Terminator Cycle Sequencing Ready Reaction Kit in both directions. Results: The DNA sequence of B*40:36 is identical to B*40:01:01 in exons 2 and 3, except for a one nucleotide substitution at residue 419 (A→T), which results in a one amino acid replacement at position 116 (Y→F; tyrosine→phenylalanine). We deduced the probable HLA haplotype in an association with B*40:36 in Taiwanese to be A*02-B*40:36-DRB1*12. Conclusion: Information on the deduced probable HLA haplotype in an association with the low incidence B*40:36 allele that we report here is of value for HLA testing laboratories for reference purposes. In addition, it can be used by stem cell transplantation donor search coordinators to determine the likelihood of finding compatible donors in unrelated bone marrow donor registries when a patient has this uncommon HLA allele.",
keywords = "Haplotype, Hematopoietic stem cell, Human leukocyte antigen, Sequence-based typing, Transplantation",
author = "Yang, {Kuo Liang} and Kao, {Reuy Ho} and Lin, {Chin Lon} and Lin, {Py Yu}",
year = "2014",
month = "1",
day = "1",
doi = "10.1016/j.tcmj.2014.07.001",
language = "English",
volume = "26",
pages = "157--160",
journal = "Tzu Chi Medical Journal",
issn = "1016-3190",
publisher = "財團法人中華民國佛教慈濟慈善事業基金會",
number = "4",

}

TY - JOUR

T1 - The deduced probable human leukocyte antigen haplotype associatedwith human leukocyte antigen low incidence allele B*40

T2 - 36 (A*02-B*40:36-DRB1*12) in Taiwanese unrelated hematopoietic bone marrow stem cell donors

AU - Yang, Kuo Liang

AU - Kao, Reuy Ho

AU - Lin, Chin Lon

AU - Lin, Py Yu

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Objective: Human leukocyte antigen (HLA)-B*40:36 is a low incidence allele in the HLA-B locus. This study reports the ethnicity of B*40:36 and the deduced probable HLA haplotype associated with HLA-B*40:36 in Taiwanese unrelated bone marrow hematopoietic stem cell donors. Materials and methods: A sequence-based typing method was employed to confirm that the low incidence allele was present. Polymerase chain reaction was performed to amplify exons 2 and 3 in the HLA-A and HLA-B loci and exon 2 in the HLA-DRB1 locus using group-specific primer sets. The amplicons were sequenced using the BigDye Terminator Cycle Sequencing Ready Reaction Kit in both directions. Results: The DNA sequence of B*40:36 is identical to B*40:01:01 in exons 2 and 3, except for a one nucleotide substitution at residue 419 (A→T), which results in a one amino acid replacement at position 116 (Y→F; tyrosine→phenylalanine). We deduced the probable HLA haplotype in an association with B*40:36 in Taiwanese to be A*02-B*40:36-DRB1*12. Conclusion: Information on the deduced probable HLA haplotype in an association with the low incidence B*40:36 allele that we report here is of value for HLA testing laboratories for reference purposes. In addition, it can be used by stem cell transplantation donor search coordinators to determine the likelihood of finding compatible donors in unrelated bone marrow donor registries when a patient has this uncommon HLA allele.

AB - Objective: Human leukocyte antigen (HLA)-B*40:36 is a low incidence allele in the HLA-B locus. This study reports the ethnicity of B*40:36 and the deduced probable HLA haplotype associated with HLA-B*40:36 in Taiwanese unrelated bone marrow hematopoietic stem cell donors. Materials and methods: A sequence-based typing method was employed to confirm that the low incidence allele was present. Polymerase chain reaction was performed to amplify exons 2 and 3 in the HLA-A and HLA-B loci and exon 2 in the HLA-DRB1 locus using group-specific primer sets. The amplicons were sequenced using the BigDye Terminator Cycle Sequencing Ready Reaction Kit in both directions. Results: The DNA sequence of B*40:36 is identical to B*40:01:01 in exons 2 and 3, except for a one nucleotide substitution at residue 419 (A→T), which results in a one amino acid replacement at position 116 (Y→F; tyrosine→phenylalanine). We deduced the probable HLA haplotype in an association with B*40:36 in Taiwanese to be A*02-B*40:36-DRB1*12. Conclusion: Information on the deduced probable HLA haplotype in an association with the low incidence B*40:36 allele that we report here is of value for HLA testing laboratories for reference purposes. In addition, it can be used by stem cell transplantation donor search coordinators to determine the likelihood of finding compatible donors in unrelated bone marrow donor registries when a patient has this uncommon HLA allele.

KW - Haplotype

KW - Hematopoietic stem cell

KW - Human leukocyte antigen

KW - Sequence-based typing

KW - Transplantation

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U2 - 10.1016/j.tcmj.2014.07.001

DO - 10.1016/j.tcmj.2014.07.001

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JF - Tzu Chi Medical Journal

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