The crystal structure of d(Ccccgggg): A new a-form variant with an extended backbone conformation

T. E. Haran, Z. Shakked, A. H. Wang, A. Rich

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98 引文 斯高帕斯(Scopus)

摘要

The crystal structure of d(CCCCGGGG) has been determined at a resolution of 2.25Ȧ. The oligomers crystallize as A-DNA duplexes occupying crystallographic two-fold axes. The backbone conformation is, in general, similar to that observed in previously reported crystal structures of A-DNA fragments, except for the central linkage, where it adopts an extended structure resulting from all trans conformation at the P-05’-C5’-C4′ bonds. This type of conformation facilitates interstrand stacking between the guanines at the C-G site. The local helix twist at this step is very small (25°) compared to an overall average of 33.5°. The unique structure of the C-G base-pair step, namely the extended backbone and the distinct stacking geometry, may be an important feature in the recognition mechanism between doublestranded DNA molecules and restriction endonucleases such as Msp I, which cuts the sequence CCGG very specifically with a rate unaffected by neighboring base pairs.

原文英語
頁(從 - 到)199-218
頁數20
期刊Journal of Biomolecular Structure and Dynamics
5
發行號2
DOIs
出版狀態已發佈 - 10月 1987
對外發佈

ASJC Scopus subject areas

  • 結構生物學
  • 分子生物學

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