The application of non-invasive apoptosis detection sensor (NIADS) on histone deacetylation inhibitor (HDACI)-induced breast cancer cell death

Kai Wen Hsu, Chien Yu Huang, Ka Wai Tam, Chun Yu Lin, Li Chi Huang, Ching Ling Lin, Wen Shyang Hsieh, Wei Ming Chi, Yu Jia Chang, Po Li Wei, Shou Tung Chen, Chia Hwa Lee

研究成果: 雜誌貢獻文章

6 引文 (Scopus)

摘要

Breast cancer is the most common malignancy in women and the second leading cause of cancer death in women. Triple negative breast cancer (TNBC) subtype is a breast cancer subset without ER (estrogen receptor), PR (progesterone receptor) and HER2 (human epidermal growth factor receptor 2) expression, limiting treatment options and presenting a poorer survival rate. Thus, we investigated whether histone deacetylation inhibitor (HDACi) could be used as potential anti-cancer therapy on breast cancer cells. In this study, we found TNBC and HER2-enriched breast cancers are extremely sensitive to Panobinostat, Belinostat of HDACi via experiments of cell viability assay, apoptotic marker identification and flow cytometry measurement. On the other hand, we developed a bioluminescence-based live cell non-invasive apoptosis detection sensor (NIADS) detection system to evaluate the quantitative and kinetic analyses of apoptotic cell death by HDAC treatment on breast cancer cells. In addition, the use of HDACi may also contribute a synergic anti-cancer effect with co-treatment of chemotherapeutic agent such as doxorubicin on TNBC cells (MDA-MB-231), but not in breast normal epithelia cells (MCF-10A), providing therapeutic benefits against breast tumor in the clinic.
原文英語
文章編號452
期刊International Journal of Molecular Sciences
19
發行號2
DOIs
出版狀態已發佈 - 二月 2 2018

指紋

apoptosis
Cell death
death
breast
Histones
inhibitors
Cell Death
cancer
Cells
Triple Negative Breast Neoplasms
Apoptosis
Breast Neoplasms
sensors
Sensors
Bioluminescence
Flow cytometry
Progesterone Receptors
Estrogen Receptors
Doxorubicin
Tumors

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

引用此文

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title = "The application of non-invasive apoptosis detection sensor (NIADS) on histone deacetylation inhibitor (HDACI)-induced breast cancer cell death",
abstract = "Breast cancer is the most common malignancy in women and the second leading cause of cancer death in women. Triple negative breast cancer (TNBC) subtype is a breast cancer subset without ER (estrogen receptor), PR (progesterone receptor) and HER2 (human epidermal growth factor receptor 2) expression, limiting treatment options and presenting a poorer survival rate. Thus, we investigated whether histone deacetylation inhibitor (HDACi) could be used as potential anti-cancer therapy on breast cancer cells. In this study, we found TNBC and HER2-enriched breast cancers are extremely sensitive to Panobinostat, Belinostat of HDACi via experiments of cell viability assay, apoptotic marker identification and flow cytometry measurement. On the other hand, we developed a bioluminescence-based live cell non-invasive apoptosis detection sensor (NIADS) detection system to evaluate the quantitative and kinetic analyses of apoptotic cell death by HDAC treatment on breast cancer cells. In addition, the use of HDACi may also contribute a synergic anti-cancer effect with co-treatment of chemotherapeutic agent such as doxorubicin on TNBC cells (MDA-MB-231), but not in breast normal epithelia cells (MCF-10A), providing therapeutic benefits against breast tumor in the clinic.",
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author = "Hsu, {Kai Wen} and Huang, {Chien Yu} and Tam, {Ka Wai} and Lin, {Chun Yu} and Huang, {Li Chi} and Lin, {Ching Ling} and Hsieh, {Wen Shyang} and Chi, {Wei Ming} and Chang, {Yu Jia} and Wei, {Po Li} and Chen, {Shou Tung} and Lee, {Chia Hwa}",
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T1 - The application of non-invasive apoptosis detection sensor (NIADS) on histone deacetylation inhibitor (HDACI)-induced breast cancer cell death

AU - Hsu, Kai Wen

AU - Huang, Chien Yu

AU - Tam, Ka Wai

AU - Lin, Chun Yu

AU - Huang, Li Chi

AU - Lin, Ching Ling

AU - Hsieh, Wen Shyang

AU - Chi, Wei Ming

AU - Chang, Yu Jia

AU - Wei, Po Li

AU - Chen, Shou Tung

AU - Lee, Chia Hwa

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AB - Breast cancer is the most common malignancy in women and the second leading cause of cancer death in women. Triple negative breast cancer (TNBC) subtype is a breast cancer subset without ER (estrogen receptor), PR (progesterone receptor) and HER2 (human epidermal growth factor receptor 2) expression, limiting treatment options and presenting a poorer survival rate. Thus, we investigated whether histone deacetylation inhibitor (HDACi) could be used as potential anti-cancer therapy on breast cancer cells. In this study, we found TNBC and HER2-enriched breast cancers are extremely sensitive to Panobinostat, Belinostat of HDACi via experiments of cell viability assay, apoptotic marker identification and flow cytometry measurement. On the other hand, we developed a bioluminescence-based live cell non-invasive apoptosis detection sensor (NIADS) detection system to evaluate the quantitative and kinetic analyses of apoptotic cell death by HDAC treatment on breast cancer cells. In addition, the use of HDACi may also contribute a synergic anti-cancer effect with co-treatment of chemotherapeutic agent such as doxorubicin on TNBC cells (MDA-MB-231), but not in breast normal epithelia cells (MCF-10A), providing therapeutic benefits against breast tumor in the clinic.

KW - HDACi

KW - Live cell non-invasive apoptosis detection sensor (NIADS)

KW - Triple negative breast cancer (TNBC)

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