Tetramethylpyrazine (TMPZ) is an active ingredient of a Chinese herbal medicine (Ligusticum wallichii Franchat). In this study, TMPZ (50-200 μM) significantly increased production of nitrate and cyclic GMP in human platelets within a 15-min incubation period. TMPZ concentration-dependently inhibited intracellular Ca2+ mobilization in human platelets stimulated by collagen (5 μg/ml). Furthermore, TMPZ concentration (50 and 200 μM)- and time (15 and 30 min)-dependently triggered endothelial-type constitutive nitric oxide synthase (ecNOS) protein expression in human platelets. These results indicated that TMPZ at micromolar concentrations stimulated nitric oxide production in human platelets via a novel mechanism that activated ecNOS protein expression. (C) 2000 Elsevier Science Inc.
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Sheu, J. R., Kan, Y. C., Hung, W. C., Lin, C-H., & Yen, M. H. (2000). The antiplatelet activity of tetramethylpyrazine is mediated through activation of NO synthase. Life Sciences, 67(8), 937-947. https://doi.org/10.1016/S0024-3205(00)00686-X