The antioxidative effect of bone morphogenetic protein-7 against high glucose-induced oxidative stress in mesangial cells

Ching Hua Yeh, Cheng Kuei Chang, Meng Fu Cheng, Hung Jung Lin, Juei Tang Cheng

研究成果: 雜誌貢獻 › 文章

28 引文 (Scopus)

摘要

Bone morphogenetic protein-7 (BMP-7) protects kidneys from diabetic nephropathy (DN), and high glucose (HG)-induced oxidative stress is involved in DN. We investigated the antioxidative ability of BMP-7 using HG-treated mesangial cells. We treated rat mesangial cells (RMCs) with recombinant human BMP-7 (rhBMP-7) and examined changes in reactive oxygen species (ROS) levels and intracellular signals in response to HG-induced oxidative stress. rhBMP-7 decreased the level of ROS in HG-treated RMCs. In contrast, lowering endogenous BMP-7 by siRNA or BMP receptor II (BMP-RII) by anti-BMP-RII antibodies increased the level of ROS in HG-treated RMCs. rhBMP-7 increased Smad-1,5,8 phosphorylation, decreased PKCζ and c-Jun N-terminal kinase (JNK) phosphorylation, and decreased fibronectin and collagen IV synthesis in HG-treated RMCs. In conclusion, we found that BMP-7 could protect mesangial cells from HG-induced oxidative stress by activating BMP-RII. The antioxidative activity of BMP-7 was primarily due to inhibition of PKCζ, JNK phosphorylation, and c-jun activation.
原文英語
頁（從 - 到）292-297
頁數6
期刊Biochemical and Biophysical Research Communications
卷382
發行號2
DOIs
出版狀態已發佈 - 五月 1 2009
對外發佈Yes

指紋

Bone Morphogenetic Protein 7
Oxidative stress
Mesangial Cells
Oxidative Stress
Glucose
Bone Morphogenetic Protein Receptors
Phosphorylation
Rats
Reactive Oxygen Species
JNK Mitogen-Activated Protein Kinases
Diabetic Nephropathies
Fibronectins
Small Interfering RNA
Phosphotransferases
Collagen
Chemical activation
Kidney
Antibodies

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

引用此文

The antioxidative effect of bone morphogenetic protein-7 against high glucose-induced oxidative stress in mesangial cells. / Yeh, Ching Hua; Chang, Cheng Kuei; Cheng, Meng Fu; Lin, Hung Jung; Cheng, Juei Tang.

於: Biochemical and Biophysical Research Communications, 卷 382, 編號 2, 01.05.2009, p. 292-297.

研究成果: 雜誌貢獻 › 文章

Yeh, CH, Chang, CK, Cheng, MF, Lin, HJ & Cheng, JT 2009, 'The antioxidative effect of bone morphogenetic protein-7 against high glucose-induced oxidative stress in mesangial cells', Biochemical and Biophysical Research Communications, 卷 382, 編號 2, 頁 292-297. https://doi.org/10.1016/j.bbrc.2009.03.011
Yeh CH, Chang CK, Cheng MF, Lin HJ, Cheng JT. The antioxidative effect of bone morphogenetic protein-7 against high glucose-induced oxidative stress in mesangial cells. Biochemical and Biophysical Research Communications. 2009 5月 1;382(2):292-297. https://doi.org/10.1016/j.bbrc.2009.03.011
Yeh, Ching Hua ; Chang, Cheng Kuei ; Cheng, Meng Fu ; Lin, Hung Jung ; Cheng, Juei Tang. / The antioxidative effect of bone morphogenetic protein-7 against high glucose-induced oxidative stress in mesangial cells. 於: Biochemical and Biophysical Research Communications. 2009 ; 卷 382, 編號 2. 頁 292-297.
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