The antioxidative effect of bone morphogenetic protein-7 against high glucose-induced oxidative stress in mesangial cells

Ching Hua Yeh, Cheng Kuei Chang, Meng Fu Cheng, Hung Jung Lin, Juei Tang Cheng

研究成果: 雜誌貢獻文章

27 引文 (Scopus)

摘要

Bone morphogenetic protein-7 (BMP-7) protects kidneys from diabetic nephropathy (DN), and high glucose (HG)-induced oxidative stress is involved in DN. We investigated the antioxidative ability of BMP-7 using HG-treated mesangial cells. We treated rat mesangial cells (RMCs) with recombinant human BMP-7 (rhBMP-7) and examined changes in reactive oxygen species (ROS) levels and intracellular signals in response to HG-induced oxidative stress. rhBMP-7 decreased the level of ROS in HG-treated RMCs. In contrast, lowering endogenous BMP-7 by siRNA or BMP receptor II (BMP-RII) by anti-BMP-RII antibodies increased the level of ROS in HG-treated RMCs. rhBMP-7 increased Smad-1,5,8 phosphorylation, decreased PKCζ and c-Jun N-terminal kinase (JNK) phosphorylation, and decreased fibronectin and collagen IV synthesis in HG-treated RMCs. In conclusion, we found that BMP-7 could protect mesangial cells from HG-induced oxidative stress by activating BMP-RII. The antioxidative activity of BMP-7 was primarily due to inhibition of PKCζ, JNK phosphorylation, and c-jun activation.
原文英語
頁(從 - 到)292-297
頁數6
期刊Biochemical and Biophysical Research Communications
382
發行號2
DOIs
出版狀態已發佈 - 五月 1 2009
對外發佈Yes

指紋

Bone Morphogenetic Protein 7
Oxidative stress
Mesangial Cells
Oxidative Stress
Glucose
Bone Morphogenetic Protein Receptors
Phosphorylation
Rats
Reactive Oxygen Species
JNK Mitogen-Activated Protein Kinases
Diabetic Nephropathies
Fibronectins
Small Interfering RNA
Phosphotransferases
Collagen
Chemical activation
Kidney
Antibodies

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Molecular Biology

引用此文

The antioxidative effect of bone morphogenetic protein-7 against high glucose-induced oxidative stress in mesangial cells. / Yeh, Ching Hua; Chang, Cheng Kuei; Cheng, Meng Fu; Lin, Hung Jung; Cheng, Juei Tang.

於: Biochemical and Biophysical Research Communications, 卷 382, 編號 2, 01.05.2009, p. 292-297.

研究成果: 雜誌貢獻文章

@article{5736797a61c1433dba69bb06c1fd9b2f,
title = "The antioxidative effect of bone morphogenetic protein-7 against high glucose-induced oxidative stress in mesangial cells",
abstract = "Bone morphogenetic protein-7 (BMP-7) protects kidneys from diabetic nephropathy (DN), and high glucose (HG)-induced oxidative stress is involved in DN. We investigated the antioxidative ability of BMP-7 using HG-treated mesangial cells. We treated rat mesangial cells (RMCs) with recombinant human BMP-7 (rhBMP-7) and examined changes in reactive oxygen species (ROS) levels and intracellular signals in response to HG-induced oxidative stress. rhBMP-7 decreased the level of ROS in HG-treated RMCs. In contrast, lowering endogenous BMP-7 by siRNA or BMP receptor II (BMP-RII) by anti-BMP-RII antibodies increased the level of ROS in HG-treated RMCs. rhBMP-7 increased Smad-1,5,8 phosphorylation, decreased PKCζ and c-Jun N-terminal kinase (JNK) phosphorylation, and decreased fibronectin and collagen IV synthesis in HG-treated RMCs. In conclusion, we found that BMP-7 could protect mesangial cells from HG-induced oxidative stress by activating BMP-RII. The antioxidative activity of BMP-7 was primarily due to inhibition of PKCζ, JNK phosphorylation, and c-jun activation.",
keywords = "BMP-7, Mesangial cells, Oxidative stress, PKCζ",
author = "Yeh, {Ching Hua} and Chang, {Cheng Kuei} and Cheng, {Meng Fu} and Lin, {Hung Jung} and Cheng, {Juei Tang}",
year = "2009",
month = "5",
day = "1",
doi = "10.1016/j.bbrc.2009.03.011",
language = "English",
volume = "382",
pages = "292--297",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier B.V.",
number = "2",

}

TY - JOUR

T1 - The antioxidative effect of bone morphogenetic protein-7 against high glucose-induced oxidative stress in mesangial cells

AU - Yeh, Ching Hua

AU - Chang, Cheng Kuei

AU - Cheng, Meng Fu

AU - Lin, Hung Jung

AU - Cheng, Juei Tang

PY - 2009/5/1

Y1 - 2009/5/1

N2 - Bone morphogenetic protein-7 (BMP-7) protects kidneys from diabetic nephropathy (DN), and high glucose (HG)-induced oxidative stress is involved in DN. We investigated the antioxidative ability of BMP-7 using HG-treated mesangial cells. We treated rat mesangial cells (RMCs) with recombinant human BMP-7 (rhBMP-7) and examined changes in reactive oxygen species (ROS) levels and intracellular signals in response to HG-induced oxidative stress. rhBMP-7 decreased the level of ROS in HG-treated RMCs. In contrast, lowering endogenous BMP-7 by siRNA or BMP receptor II (BMP-RII) by anti-BMP-RII antibodies increased the level of ROS in HG-treated RMCs. rhBMP-7 increased Smad-1,5,8 phosphorylation, decreased PKCζ and c-Jun N-terminal kinase (JNK) phosphorylation, and decreased fibronectin and collagen IV synthesis in HG-treated RMCs. In conclusion, we found that BMP-7 could protect mesangial cells from HG-induced oxidative stress by activating BMP-RII. The antioxidative activity of BMP-7 was primarily due to inhibition of PKCζ, JNK phosphorylation, and c-jun activation.

AB - Bone morphogenetic protein-7 (BMP-7) protects kidneys from diabetic nephropathy (DN), and high glucose (HG)-induced oxidative stress is involved in DN. We investigated the antioxidative ability of BMP-7 using HG-treated mesangial cells. We treated rat mesangial cells (RMCs) with recombinant human BMP-7 (rhBMP-7) and examined changes in reactive oxygen species (ROS) levels and intracellular signals in response to HG-induced oxidative stress. rhBMP-7 decreased the level of ROS in HG-treated RMCs. In contrast, lowering endogenous BMP-7 by siRNA or BMP receptor II (BMP-RII) by anti-BMP-RII antibodies increased the level of ROS in HG-treated RMCs. rhBMP-7 increased Smad-1,5,8 phosphorylation, decreased PKCζ and c-Jun N-terminal kinase (JNK) phosphorylation, and decreased fibronectin and collagen IV synthesis in HG-treated RMCs. In conclusion, we found that BMP-7 could protect mesangial cells from HG-induced oxidative stress by activating BMP-RII. The antioxidative activity of BMP-7 was primarily due to inhibition of PKCζ, JNK phosphorylation, and c-jun activation.

KW - BMP-7

KW - Mesangial cells

KW - Oxidative stress

KW - PKCζ

UR - http://www.scopus.com/inward/record.url?scp=63549125875&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=63549125875&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2009.03.011

DO - 10.1016/j.bbrc.2009.03.011

M3 - Article

VL - 382

SP - 292

EP - 297

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -