The antihypertensive effect of stevioside derivative isosteviol in spontaneously hypertensive rats

研究成果: 雜誌貢獻文章

28 引文 (Scopus)

摘要

Background: Hypertension is one of the most common disease entity causing major mortality. Isosteviol (IST) is the derivative from stevioside. Stevioside is a sweet-tasting glycoside existed in the leaves of the herb Stevia rebaudiana that has been using widely in Japan as sugar substitute. Stevioside has been proved to have hypotensive effect. This study investigated the antihypertensive effect of isosteviol in spontaneously hypertensive rats (SHR). Methods: Isosteviol synthesized from stevioside was employed to inject intraperitoreally into SHR by dose-dependent manner. Isolated aortic strips and vascular smooth muscle cells were employed to evaluate the mechanism of hypotension. Results: IST (25 mg/kg) given intraperitoneally decreased mean artery blood pressure by 12 mmHg. In isolated aortic rings from rats, IST could dose-dependently relaxed vasopressin-induced vasoconstriction. IST had no effect on phenylephrine- induced vasoconstriction that produced mainly due to intracellular calcium (Ca2+) release. Also, IST failed to modify the vasopressin-induced vasoconstriction in Ca2+-free medium. The results indicate that IST has vasorelaxatory effect via an inhibition of Ca2+ influx. Reduction in the vasopressin-induced vasoconstriction by IST was also obtained in the absence of endothelium, showing this pharmacological effect of IST was not related to nitric oxide and/or endothelium system. Conclusions: Isosteviol (IST) the new derivative from stevioside, has remarkable antihypertensive effect as its parent compound in SHR. The mechanism of hypotension is probably through calcium influx inhibition in vascular smooth muscle cells.
原文英語
頁(從 - 到)133-140
頁數8
期刊Acta Cardiologica Sinica
17
發行號3
出版狀態已發佈 - 2001

指紋

Inbred SHR Rats
Antihypertensive Agents
Vasoconstriction
Vasopressins
Vascular Smooth Muscle
Hypotension
Smooth Muscle Myocytes
Endothelium
stevioside
isosteviol
Stevia
Calcium
Sweetening Agents
Phenylephrine
Glycosides
Japan
Nitric Oxide
Arteries
Pharmacology
Blood Pressure

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

引用此文

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title = "The antihypertensive effect of stevioside derivative isosteviol in spontaneously hypertensive rats",
abstract = "Background: Hypertension is one of the most common disease entity causing major mortality. Isosteviol (IST) is the derivative from stevioside. Stevioside is a sweet-tasting glycoside existed in the leaves of the herb Stevia rebaudiana that has been using widely in Japan as sugar substitute. Stevioside has been proved to have hypotensive effect. This study investigated the antihypertensive effect of isosteviol in spontaneously hypertensive rats (SHR). Methods: Isosteviol synthesized from stevioside was employed to inject intraperitoreally into SHR by dose-dependent manner. Isolated aortic strips and vascular smooth muscle cells were employed to evaluate the mechanism of hypotension. Results: IST (25 mg/kg) given intraperitoneally decreased mean artery blood pressure by 12 mmHg. In isolated aortic rings from rats, IST could dose-dependently relaxed vasopressin-induced vasoconstriction. IST had no effect on phenylephrine- induced vasoconstriction that produced mainly due to intracellular calcium (Ca2+) release. Also, IST failed to modify the vasopressin-induced vasoconstriction in Ca2+-free medium. The results indicate that IST has vasorelaxatory effect via an inhibition of Ca2+ influx. Reduction in the vasopressin-induced vasoconstriction by IST was also obtained in the absence of endothelium, showing this pharmacological effect of IST was not related to nitric oxide and/or endothelium system. Conclusions: Isosteviol (IST) the new derivative from stevioside, has remarkable antihypertensive effect as its parent compound in SHR. The mechanism of hypotension is probably through calcium influx inhibition in vascular smooth muscle cells.",
keywords = "Calcium influx inhibition, Isosteviol, Spontaneously hypertensive rat, Stevioside",
author = "Liu, {J. C.} and Kao, {P. F.} and Hsieh, {M. H.} and Chen, {Y. J.} and P. Chan",
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T1 - The antihypertensive effect of stevioside derivative isosteviol in spontaneously hypertensive rats

AU - Liu, J. C.

AU - Kao, P. F.

AU - Hsieh, M. H.

AU - Chen, Y. J.

AU - Chan, P.

PY - 2001

Y1 - 2001

N2 - Background: Hypertension is one of the most common disease entity causing major mortality. Isosteviol (IST) is the derivative from stevioside. Stevioside is a sweet-tasting glycoside existed in the leaves of the herb Stevia rebaudiana that has been using widely in Japan as sugar substitute. Stevioside has been proved to have hypotensive effect. This study investigated the antihypertensive effect of isosteviol in spontaneously hypertensive rats (SHR). Methods: Isosteviol synthesized from stevioside was employed to inject intraperitoreally into SHR by dose-dependent manner. Isolated aortic strips and vascular smooth muscle cells were employed to evaluate the mechanism of hypotension. Results: IST (25 mg/kg) given intraperitoneally decreased mean artery blood pressure by 12 mmHg. In isolated aortic rings from rats, IST could dose-dependently relaxed vasopressin-induced vasoconstriction. IST had no effect on phenylephrine- induced vasoconstriction that produced mainly due to intracellular calcium (Ca2+) release. Also, IST failed to modify the vasopressin-induced vasoconstriction in Ca2+-free medium. The results indicate that IST has vasorelaxatory effect via an inhibition of Ca2+ influx. Reduction in the vasopressin-induced vasoconstriction by IST was also obtained in the absence of endothelium, showing this pharmacological effect of IST was not related to nitric oxide and/or endothelium system. Conclusions: Isosteviol (IST) the new derivative from stevioside, has remarkable antihypertensive effect as its parent compound in SHR. The mechanism of hypotension is probably through calcium influx inhibition in vascular smooth muscle cells.

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KW - Calcium influx inhibition

KW - Isosteviol

KW - Spontaneously hypertensive rat

KW - Stevioside

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