Terbinafine inhibits oral squamous cell carcinoma growth through anti-cancer cell proliferation and anti-angiogenesis

Ming Hsien Chien, Tong Sheng Lee, Chieh Kao, Shun Fa Yang, Wen Sen Lee

研究成果: 雜誌貢獻文章

11 引文 (Scopus)

摘要

Terbinafine (TB), an oral antifungal agent used in the treatment of superficial mycosis, has been reported to exert an anti-tumor effect in various cancer cells. However, the effect of TB on oral cancer has not been evaluated. Herein we demonstrate that TB (0-60μM) concentration-dependently decreased cell number in cultured human oral squamous cell carcinoma (OSCC), KB cells. The anti-proliferation effect of TB was also observed in two other OSCC cell lines, SAS and SCC 15. TB (60μM) was not cytotoxic and its inhibition on KB cell growth was reversible. [ 3H]thymidine incorporation and flow cytometric analyses revealed that TB-inhibited DNA synthesis and induced the G0/G1 cell-cycle arrest. The TB-induced cell-cycle arrest occurred when the cyclin-dependent kinase 2 activity was inhibited just as the protein levels of p21 cip1 and p27 kip1 were increased. The TB-induced G0/G1 cell-cycle arrest was completely blocked when the expressions of p21 cip1 and p27 kip1 were knocked-down together. Taken together, these results suggest that the p21 cip1- and p27 kip1-associated signaling pathways might be involved in the TB-induced anti-proliferation in KB cells. In vivo, TB (50mg/kg, i.p.) significantly inhibited the KB tumor size. In these TB-treated tumors, increases in the levels of p21 cip1 and p27 kip1 protein and decreases in the number of proliferating cell nuclear antigen-positive cells and the microvessel density were observed. These findings demonstrate for the first time that TB might have potential to serve as a therapeutic tool in the treatment of oral cancer.

原文英語
頁(從 - 到)389-399
頁數11
期刊Molecular Carcinogenesis
51
發行號5
DOIs
出版狀態已發佈 - 五月 2012

指紋

terbinafine
Squamous Cell Carcinoma
Cell Proliferation
Growth
Neoplasms
KB Cells
G1 Phase Cell Cycle Checkpoints
Mouth Neoplasms
Cell Count

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology

引用此文

Terbinafine inhibits oral squamous cell carcinoma growth through anti-cancer cell proliferation and anti-angiogenesis. / Chien, Ming Hsien; Lee, Tong Sheng; Kao, Chieh; Yang, Shun Fa; Lee, Wen Sen.

於: Molecular Carcinogenesis, 卷 51, 編號 5, 05.2012, p. 389-399.

研究成果: 雜誌貢獻文章

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abstract = "Terbinafine (TB), an oral antifungal agent used in the treatment of superficial mycosis, has been reported to exert an anti-tumor effect in various cancer cells. However, the effect of TB on oral cancer has not been evaluated. Herein we demonstrate that TB (0-60μM) concentration-dependently decreased cell number in cultured human oral squamous cell carcinoma (OSCC), KB cells. The anti-proliferation effect of TB was also observed in two other OSCC cell lines, SAS and SCC 15. TB (60μM) was not cytotoxic and its inhibition on KB cell growth was reversible. [ 3H]thymidine incorporation and flow cytometric analyses revealed that TB-inhibited DNA synthesis and induced the G0/G1 cell-cycle arrest. The TB-induced cell-cycle arrest occurred when the cyclin-dependent kinase 2 activity was inhibited just as the protein levels of p21 cip1 and p27 kip1 were increased. The TB-induced G0/G1 cell-cycle arrest was completely blocked when the expressions of p21 cip1 and p27 kip1 were knocked-down together. Taken together, these results suggest that the p21 cip1- and p27 kip1-associated signaling pathways might be involved in the TB-induced anti-proliferation in KB cells. In vivo, TB (50mg/kg, i.p.) significantly inhibited the KB tumor size. In these TB-treated tumors, increases in the levels of p21 cip1 and p27 kip1 protein and decreases in the number of proliferating cell nuclear antigen-positive cells and the microvessel density were observed. These findings demonstrate for the first time that TB might have potential to serve as a therapeutic tool in the treatment of oral cancer.",
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AU - Lee, Wen Sen

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N2 - Terbinafine (TB), an oral antifungal agent used in the treatment of superficial mycosis, has been reported to exert an anti-tumor effect in various cancer cells. However, the effect of TB on oral cancer has not been evaluated. Herein we demonstrate that TB (0-60μM) concentration-dependently decreased cell number in cultured human oral squamous cell carcinoma (OSCC), KB cells. The anti-proliferation effect of TB was also observed in two other OSCC cell lines, SAS and SCC 15. TB (60μM) was not cytotoxic and its inhibition on KB cell growth was reversible. [ 3H]thymidine incorporation and flow cytometric analyses revealed that TB-inhibited DNA synthesis and induced the G0/G1 cell-cycle arrest. The TB-induced cell-cycle arrest occurred when the cyclin-dependent kinase 2 activity was inhibited just as the protein levels of p21 cip1 and p27 kip1 were increased. The TB-induced G0/G1 cell-cycle arrest was completely blocked when the expressions of p21 cip1 and p27 kip1 were knocked-down together. Taken together, these results suggest that the p21 cip1- and p27 kip1-associated signaling pathways might be involved in the TB-induced anti-proliferation in KB cells. In vivo, TB (50mg/kg, i.p.) significantly inhibited the KB tumor size. In these TB-treated tumors, increases in the levels of p21 cip1 and p27 kip1 protein and decreases in the number of proliferating cell nuclear antigen-positive cells and the microvessel density were observed. These findings demonstrate for the first time that TB might have potential to serve as a therapeutic tool in the treatment of oral cancer.

AB - Terbinafine (TB), an oral antifungal agent used in the treatment of superficial mycosis, has been reported to exert an anti-tumor effect in various cancer cells. However, the effect of TB on oral cancer has not been evaluated. Herein we demonstrate that TB (0-60μM) concentration-dependently decreased cell number in cultured human oral squamous cell carcinoma (OSCC), KB cells. The anti-proliferation effect of TB was also observed in two other OSCC cell lines, SAS and SCC 15. TB (60μM) was not cytotoxic and its inhibition on KB cell growth was reversible. [ 3H]thymidine incorporation and flow cytometric analyses revealed that TB-inhibited DNA synthesis and induced the G0/G1 cell-cycle arrest. The TB-induced cell-cycle arrest occurred when the cyclin-dependent kinase 2 activity was inhibited just as the protein levels of p21 cip1 and p27 kip1 were increased. The TB-induced G0/G1 cell-cycle arrest was completely blocked when the expressions of p21 cip1 and p27 kip1 were knocked-down together. Taken together, these results suggest that the p21 cip1- and p27 kip1-associated signaling pathways might be involved in the TB-induced anti-proliferation in KB cells. In vivo, TB (50mg/kg, i.p.) significantly inhibited the KB tumor size. In these TB-treated tumors, increases in the levels of p21 cip1 and p27 kip1 protein and decreases in the number of proliferating cell nuclear antigen-positive cells and the microvessel density were observed. These findings demonstrate for the first time that TB might have potential to serve as a therapeutic tool in the treatment of oral cancer.

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