TEMPO-oxidized sacchachitin nanofibers (TOSCNFs) combined with platelet-rich plasma (PRP) for management of dry eye syndrome

Hong Liang Lin, Ting Huan Wu, Hsiu O. Ho, Fang Ching Chao, Meng-Huang, Wu Der Zen Liu, Ling Chun Chen, Ming Thau Sheu

研究成果: 雜誌貢獻文章

摘要

Introduction: In this study, the combination of TEMPO-oxidized sacchachitin nanofibers (TOSCNFs) with chitosan-activated platelet-rich plasma (cPRP) was evaluated for remedying dry eye syndrome (DES). Methods: TOSCNFs, designated T050SC, were generated. T050SC combined with chitosan-activated (cPRP) was formulated as eye drops for application for severe DES. To evaluate the effects of cPRP and TOSCNFs on the repair of corneal injury, in vitro studies were conducted using Statens Seruminstitut rabbit corneal (SIRC) epithelial cells for cell proliferation and cell migration assays, and a severe DES animal model using rabbits was established with benzalkonium chloride (BAC) treatment for the evaluation. Results: Results showed that the optimal eye formulation contained PRP activated by 350 μg/mL of the low-molecular-weight chitosan group (L3) combined with 300 μg/mL TO50SC (L3+T050SC). In the WST-1 cell-proliferation assay, L3 and L3+TO50SC significantly increased Statens SIRC cell proliferation after 24 hrs of incubation. In the SIRC cell migration assay, the L3+TO50SC group showed a wound-healing efficiency of 89% after 24-hr treatment. After 5 days of treatment, Schirmer’s test results did not simulate the dry eye animal model. Typical cornea appearance and eye fluorescein staining results showed that the L3 group had the best effect on improving cornea haze and epithelial damage. Conclusion: This study has determined that TOSCNFs effectively promoted the healing effect on severe cases of corneal damage, and also might enhance the clinical application and medical potential of PRP in ophthalmology.

原文英語
頁(從 - 到)1721-1730
頁數10
期刊International Journal of Nanomedicine
15
DOIs
出版狀態已發佈 - 一月 1 2020

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

指紋 深入研究「TEMPO-oxidized sacchachitin nanofibers (TOSCNFs) combined with platelet-rich plasma (PRP) for management of dry eye syndrome」主題。共同形成了獨特的指紋。

  • 引用此