Targeted methylation of CMV and E1A viral promoters

Chia Chen Hsu, Hsin Pai Li, Yu Hung Hung, Yu Wei Leu, Wu Hsiung Wu, Feng Sheng Wang, Kuan Der Lee, Pey Jium Chang, Chi Sheng Wu, Yen Jung Lu, Tim H M Huang, Yu Sun Chang, Shu Huei Hsiao

研究成果: 雜誌貢獻文章同行評審

48 引文 斯高帕斯(Scopus)

摘要

DNA methylation is a gene-silencing and host defense system that can down-regulate viral gene expression in mammalian cells. An established targeted DNA methylation method was used to demonstrate that genome-integrated CMV and adenovirus type 5 E1A promoters were hypermethylated after MCF7 and HEK293 cells were transfected with in vitro methylated viral promoter fragments. In both cases, the targeted methylation-induced gene silencing could be reversed by addition of 5-aza-2'-deoxycytidine, confirming that the CMV and E1A promoters are regulated by DNA methylation. The kinetics of the targeted DNA methylation was determined using a reporter system in live cells. In conclusion, targeted DNA methylation is able to efficiently silence susceptible viral promoters and provides an alternative strategy to study the impact of loci-specific DNA methylation in viral gene expression.
原文英語
頁(從 - 到)228-234
頁數7
期刊Biochemical and Biophysical Research Communications
402
發行號2
DOIs
出版狀態已發佈 - 十一月 12 2010
對外發佈

ASJC Scopus subject areas

  • 生物化學
  • 生物物理學
  • 細胞生物學
  • 分子生物學

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