Targeted anti-apoptosis activity for ovarian protection against chemotherapy-induced ovarian gonadotoxicity

Shun Jen Tan, Li Jen Lee, Chii Ruey Tzeng, Chia-Woei Wang, Ming I. Hsu, Chi Huang Chen

研究成果: 雜誌貢獻文章同行評審

18 引文 斯高帕斯(Scopus)

摘要

Chemotherapy damages the reproductive system by enhancing apoptosis, and evidence suggests that targeted anti-apoptotic therapy may preserve fertility in patients receiving chemotherapy. To investigate the protective effect of sphingosine-1-phosphate (S1P) on chemotherapeutic agent-induced ovarian gonadotoxicity, busulfan-treated female mice were pre-treated with low (0.5 mM) and high (2.0 mM) doses of S1P or vehicle 1 h before busulfan injection. In the S1P groups, each mouse was injected with low-dose S1P in one ovary and high-dose S1P in the contralateral ovary. Four weeks later, the ovaries were removed for histological and biochemical examinations. Caspase 3 immunoreactivity was greater in mice treated with busulfan compared with mice pre-treated with S1P, in which more primordial follicles were observed (P <0.05). The mRNA level of anti-Müllerian hormone was higher in mice pre-treated with S1P than those that received busulfan only, indicating a better ovarian function in mice pre-treated with S1P. No difference was observed in the levels of growth differentiation factor-9 among all groups. In conclusion, S1P protects primordial follicles from chemotherapy-induced gonadotoxicity, and may partially preserve ovarian function.
原文英語
頁(從 - 到)612-620
頁數9
期刊Reproductive BioMedicine Online
29
發行號5
DOIs
出版狀態已發佈 - 十一月 1 2014

ASJC Scopus subject areas

  • 生殖醫學
  • 婦產科
  • 發展生物學

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