Tanshinone IIA Induces Heme Oxygenase 1 Expression and Inhibits Cyclic Strain-Induced Interleukin 8 Expression in Vascular Endothelial Cells

Shaowei Zhuang, Tzu-Hurng Cheng, Nang Lang Shih, Ju Chi Liu, Jin Jer Chen, Hong Jye Hong, Paul Chan

研究成果: 雜誌貢獻文章

12 引文 (Scopus)

摘要

Tanshinone IIA is the main effective component of Salvia miltiorrhiza, known as "Danshen," which has been used in many therapeutic remedies in traditional Chinese medicine. However, the direct effects of tanshinone IIA on vascular endothelial cells have not yet been fully described. In the present study, we demonstrated that tanshinone IIA increased heme oxygenase-1 (HO-1) expression in human umbilical vein endothelial cells. Western blot analyses and experiments with specific inhibitors indicated tanshinone IIA enhanced HO-1 expression through the activation of phosphoinositide 3-kinase (PI3K)/Akt and the subsequent induction of nuclear factor erythroid 2-related factor 2 (Nrf2) nuclear translocation. In addition, tanshinone IIA inhibited cyclic strain induced interleukin-8 (IL-8) expression. HO-1 silencing significantly abrogated the repressive effects of tanshinone IIA on strain-induced IL-8 expression, which suggests HO-1 has a role in mediating the effects of tanshinone IIA. This study reports for the first time that tanshinone IIA inhibits cyclic strain-induced IL-8 expression via the induction of HO-1 in endothelial cells, providing valuable new insight into the molecular pathways that may contribute to the effects of tanshinone IIA.
原文英語
頁(從 - 到)377-388
頁數12
期刊American Journal of Chinese Medicine
44
發行號2
DOIs
出版狀態已發佈 - 四月 1 2016

指紋

Heme Oxygenase-1
Interleukin-8
Endothelial Cells
Salvia miltiorrhiza
tanshinone
1-Phosphatidylinositol 4-Kinase
Chinese Traditional Medicine
Human Umbilical Vein Endothelial Cells
Western Blotting

ASJC Scopus subject areas

  • Complementary and alternative medicine

引用此文

Tanshinone IIA Induces Heme Oxygenase 1 Expression and Inhibits Cyclic Strain-Induced Interleukin 8 Expression in Vascular Endothelial Cells. / Zhuang, Shaowei; Cheng, Tzu-Hurng; Shih, Nang Lang; Liu, Ju Chi; Chen, Jin Jer; Hong, Hong Jye; Chan, Paul.

於: American Journal of Chinese Medicine, 卷 44, 編號 2, 01.04.2016, p. 377-388.

研究成果: 雜誌貢獻文章

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abstract = "Tanshinone IIA is the main effective component of Salvia miltiorrhiza, known as {"}Danshen,{"} which has been used in many therapeutic remedies in traditional Chinese medicine. However, the direct effects of tanshinone IIA on vascular endothelial cells have not yet been fully described. In the present study, we demonstrated that tanshinone IIA increased heme oxygenase-1 (HO-1) expression in human umbilical vein endothelial cells. Western blot analyses and experiments with specific inhibitors indicated tanshinone IIA enhanced HO-1 expression through the activation of phosphoinositide 3-kinase (PI3K)/Akt and the subsequent induction of nuclear factor erythroid 2-related factor 2 (Nrf2) nuclear translocation. In addition, tanshinone IIA inhibited cyclic strain induced interleukin-8 (IL-8) expression. HO-1 silencing significantly abrogated the repressive effects of tanshinone IIA on strain-induced IL-8 expression, which suggests HO-1 has a role in mediating the effects of tanshinone IIA. This study reports for the first time that tanshinone IIA inhibits cyclic strain-induced IL-8 expression via the induction of HO-1 in endothelial cells, providing valuable new insight into the molecular pathways that may contribute to the effects of tanshinone IIA.",
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