T-type calcium current in electrical activity of cardiomyocytes isolated from rabbit pulmonary vein

Yao Chang Chen, Shih Ann Chen, Yi Jen Chen, Ching Tai Tai, Paul Chan, Cheng I. Lin

研究成果: 雜誌貢獻文章

43 引文 斯高帕斯(Scopus)

摘要

Introduction: Pulmonary veins (PVs) are known to initiate paroxysmal atrial fibrillation. T-type calcium current (ICa-T) has a role in normal and abnormal automaticity of cardiomyocytes. The aim of this study was to evaluate whether ICa-T contributes to PV electrical activity. Methods and Results: By whole-cell clamp techniques in rabbit myocytes, I Ca-T was identified in 12 (39%) of 31 PV cardiomyocytes with pacemaker activity, 2 (9%) of 23 PV cardiomyocytes without pacemaker activity, and 2 (15%) of 13 atrial myocytes (P <0.05). Maximum ICa-L and ICa-T densities from PV cardiomyocytes with pacemaker activity were 6.87 ± 2.17 pA/pF and 1.38 ± 0.69 pA/pF, respectively. Nickel (40 μM) decreased the spontaneous activity in 5 (36%) of 14 PV cardiomyocytes (3.1 ± 0.6 Hz vs 2.2 ± 0.5 Hz, P <0.05), reduced the amplitudes of delayed afterdepolarization from 13 ± 1 mV to 7 ± 1 mV (n = 4, P <0.05) and inhibited transient inward currents from 1.2 ± 0.2 pA/pF to 0.7 ± 0.1 pA/pF (n = 11, P <0.01). Conclusions: We conclude that ICa-T contributes to PV pacemaker activity and triggered activity, which are of functional importance in PV arrhythmogenesis.

原文英語
頁(從 - 到)567-571
頁數5
期刊Journal of Cardiovascular Electrophysiology
15
發行號5
出版狀態已發佈 - 五月 2004

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology

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