Synthetic double-stranded RNA induces interleukin-32 in bronchial epithelial cells

Kyoko Ota, Mio Kawaguchi, Junichi Fujita, Fumio Kokubu, Shau Ku Huang, Yuko Morishima, Satoshi Matsukura, Masatsugu Kurokawa, Yukio Ishii, Hiroaki Satoh, Tohru Sakamoto, Nobuyuki Hizawa

研究成果: 雜誌貢獻文章同行評審

12 引文 斯高帕斯(Scopus)


Objective: Interleukin (IL)-32 is a novel cytokine and is involved in the pathogenesis of various inflammatory diseases, including asthma and COPD. However, the regulatory mechanisms of IL-32 expression and its precise pathogenic role remain to be defined. Given that viral infections are known to potentially cause and exacerbate airway inflammation, in this study, we investigated the expression of IL-32 induced by synthetic double-stranded (ds) RNA, and its signaling mechanisms involved. Methods: Bronchial epithelial cells were stimulated with synthetic dsRNA poly I:C. The levels of IL-32 expression were analyzed using real-time PCR and ELISA. The involvement of transforming growth factor β-activated kinase 1 (TAK1) and a subunit of nuclear factor-κB (NF-κB), p65 was determined by western blot analyses. TAK1 inhibitor, 5Z-7-Oxozeaenol and NF-κB inhibitor, BAY 11-7082 were added to the culture to identify key signaling events leading to the expression of IL-32. Finally, the effect of short interfering RNAs (siRNAs) targeting TAK1 and p65 was investigated. Results: dsRNA significantly induced IL-32 gene and protein expression, concomitant with activation of TAK1 and p65. Pretreatment of 5Z-7-Oxozeaenol diminished dsRNA-induced phosphorylation of NF-κB. Both 5Z-7-Oxozeaenol and BAY 11-7082 significantly abrogated dsRNA-induced IL-32 production. Moreover, transfection of the cells with siRNAs targeting TAK1 and p65 inhibited the expression of IL-32. Conclusions: The expression of IL-32 is induced by dsRNA via the TAK1-NF-κB signaling pathway in bronchial epithelial cells. IL-32 is involved in the pathogenesis of airway inflammation, and may be a novel therapeutic target for airway inflammatory diseases.

頁(從 - 到)335-343
期刊Experimental Lung Research
出版狀態已發佈 - 8月 1 2015

ASJC Scopus subject areas

  • 分子生物學
  • 肺和呼吸系統醫學
  • 臨床生物化學


深入研究「Synthetic double-stranded RNA induces interleukin-32 in bronchial epithelial cells」主題。共同形成了獨特的指紋。