Synthesized Peptides from Yam Dioscorin Hydrolysis in Silico Exhibit Dipeptidyl Peptidase-IV Inhibitory Activities and Oral Glucose Tolerance Improvements in Normal Mice

Yin Shiou Lin, Chuan Hsiao Han, Shyr Yi Lin, Wen Chi Hou

研究成果: 雜誌貢獻文章

5 引文 (Scopus)

摘要

RRDY, RL, and DPF were the top 3 of 21 peptides for inhibitions against dipeptidyl peptidase-IV (DPP-IV) from the pepsin hydrolysis of yam dioscorin in silico and were further investigated in a proof-of-concept study in normal ICR mice for regulating glucose metabolism by the oral glucose tolerance test (OGTT). The sample or sitagliptin (positive control) was orally administered by a feeding gauge; 30 min later, the glucose loads (2.5 g/kg) were performed. RRDY, yam dioscorin, or sitagliptin preload, but not DPF, lowered the area under the curve (AUC0-120) of blood glucose and DPP-IV activity and elevated the AUC0-120 of blood insulin, which showed significant differences compared to control (P < 0.05 or 0.001). These results suggested that RRDY and yam dioscorin might be beneficial in glycemic control in normal mice and need further investigations in diabetic animal models.

原文英語
頁(從 - 到)6451-6458
頁數8
期刊Journal of Agricultural and Food Chemistry
64
發行號33
DOIs
出版狀態已發佈 - 八月 24 2016

指紋

Dioscorea
Dipeptidyl Peptidase 4
yams
glucose tolerance
Glucose Tolerance Test
Computer Simulation
Hydrolysis
mouth
hydrolysis
peptides
Glucose
Peptides
mice
Inbred ICR Mouse
glycemic control
glucose
Pepsin A
pepsin
gauges
Metabolism

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

引用此文

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abstract = "RRDY, RL, and DPF were the top 3 of 21 peptides for inhibitions against dipeptidyl peptidase-IV (DPP-IV) from the pepsin hydrolysis of yam dioscorin in silico and were further investigated in a proof-of-concept study in normal ICR mice for regulating glucose metabolism by the oral glucose tolerance test (OGTT). The sample or sitagliptin (positive control) was orally administered by a feeding gauge; 30 min later, the glucose loads (2.5 g/kg) were performed. RRDY, yam dioscorin, or sitagliptin preload, but not DPF, lowered the area under the curve (AUC0-120) of blood glucose and DPP-IV activity and elevated the AUC0-120 of blood insulin, which showed significant differences compared to control (P < 0.05 or 0.001). These results suggested that RRDY and yam dioscorin might be beneficial in glycemic control in normal mice and need further investigations in diabetic animal models.",
keywords = "blood insulin, blood sugar, dipeptidyl peptidase-IV (DPP-IV), oral glucose tolerance test (OGTT), sitagliptin, yam dioscorin",
author = "Lin, {Yin Shiou} and Han, {Chuan Hsiao} and Lin, {Shyr Yi} and Hou, {Wen Chi}",
year = "2016",
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AU - Lin, Yin Shiou

AU - Han, Chuan Hsiao

AU - Lin, Shyr Yi

AU - Hou, Wen Chi

PY - 2016/8/24

Y1 - 2016/8/24

N2 - RRDY, RL, and DPF were the top 3 of 21 peptides for inhibitions against dipeptidyl peptidase-IV (DPP-IV) from the pepsin hydrolysis of yam dioscorin in silico and were further investigated in a proof-of-concept study in normal ICR mice for regulating glucose metabolism by the oral glucose tolerance test (OGTT). The sample or sitagliptin (positive control) was orally administered by a feeding gauge; 30 min later, the glucose loads (2.5 g/kg) were performed. RRDY, yam dioscorin, or sitagliptin preload, but not DPF, lowered the area under the curve (AUC0-120) of blood glucose and DPP-IV activity and elevated the AUC0-120 of blood insulin, which showed significant differences compared to control (P < 0.05 or 0.001). These results suggested that RRDY and yam dioscorin might be beneficial in glycemic control in normal mice and need further investigations in diabetic animal models.

AB - RRDY, RL, and DPF were the top 3 of 21 peptides for inhibitions against dipeptidyl peptidase-IV (DPP-IV) from the pepsin hydrolysis of yam dioscorin in silico and were further investigated in a proof-of-concept study in normal ICR mice for regulating glucose metabolism by the oral glucose tolerance test (OGTT). The sample or sitagliptin (positive control) was orally administered by a feeding gauge; 30 min later, the glucose loads (2.5 g/kg) were performed. RRDY, yam dioscorin, or sitagliptin preload, but not DPF, lowered the area under the curve (AUC0-120) of blood glucose and DPP-IV activity and elevated the AUC0-120 of blood insulin, which showed significant differences compared to control (P < 0.05 or 0.001). These results suggested that RRDY and yam dioscorin might be beneficial in glycemic control in normal mice and need further investigations in diabetic animal models.

KW - blood insulin

KW - blood sugar

KW - dipeptidyl peptidase-IV (DPP-IV)

KW - oral glucose tolerance test (OGTT)

KW - sitagliptin

KW - yam dioscorin

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