Synergistic effect of HIF-1α and FoxO3a trigger cardiomyocyte apoptosis under hyperglycemic ischemia condition

Ya Fang Chen, Sudhir Pandey, Cecilia Hsuan Day, Yu Feng Chen, Ai Zhi Jiang, Tsung Jung Ho, Ray Jade Chen, Vijaya V. Padma, Wei Wen Kuo, Chih Yang Huang

研究成果: 雜誌貢獻文章

17 引文 斯高帕斯(Scopus)

摘要

Cardiomyocyte death is an important pathogenic feature of ischemia and heart failure. Through this study, we showed the synergistic role of HIF-1α and FoxO3a in cardiomyocyte apoptosis subjected to hypoxia plus elevated glucose levels. Using gene specific small interfering RNAs (siRNA), semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), Western blot, immunofluorescence, nuclear and cytosolic localization and TUNEL assay techniques, we determined that combined function of HIF-1α and FoxO3a under high glucose plus hypoxia condition lead to enhanced expression of BNIP3 inducing cardiomyocyte death. Our results highlighted the importance of the synergistic role of HIF-1α and FoxO3a in cardiomyocyte death which may add insight into therapeutic approaches to pathophysiology associated with ischemic diabetic cardiomyopathies.
原文英語
頁(從 - 到)3660-3671
期刊Journal of Cellular Physiology
233
發行號4
DOIs
出版狀態已發佈 - 四月 2018

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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    Chen, Y. F., Pandey, S., Day, C. H., Chen, Y. F., Jiang, A. Z., Ho, T. J., Chen, R. J., Padma, V. V., Kuo, W. W., & Huang, C. Y. (2018). Synergistic effect of HIF-1α and FoxO3a trigger cardiomyocyte apoptosis under hyperglycemic ischemia condition. Journal of Cellular Physiology, 233(4), 3660-3671. https://doi.org/10.1002/jcp.26235