Cardiomyocyte death is an important pathogenic feature of ischemia and heart failure. Through this study, we showed the synergistic role of HIF-1α and FoxO3a in cardiomyocyte apoptosis subjected to hypoxia plus elevated glucose levels. Using gene specific small interfering RNAs (siRNA), semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), Western blot, immunofluorescence, nuclear and cytosolic localization and TUNEL assay techniques, we determined that combined function of HIF-1α and FoxO3a under high glucose plus hypoxia condition lead to enhanced expression of BNIP3 inducing cardiomyocyte death. Our results highlighted the importance of the synergistic role of HIF-1α and FoxO3a in cardiomyocyte death which may add insight into therapeutic approaches to pathophysiology associated with ischemic diabetic cardiomyopathies.
ASJC Scopus subject areas
- Clinical Biochemistry
- Cell Biology
Chen, Y. F., Pandey, S., Day, C. H., Chen, Y. F., Jiang, A. Z., Ho, T. J., Chen, R. J., Padma, V. V., Kuo, W. W., & Huang, C. Y. (2018). Synergistic effect of HIF-1α and FoxO3a trigger cardiomyocyte apoptosis under hyperglycemic ischemia condition. Journal of Cellular Physiology, 233(4), 3660-3671. https://doi.org/10.1002/jcp.26235