Suppressive effects of 3-O-methylquercetin on ovalbumin-induced airway hyperresponsiveness

Wun-Chang Ko, Chwen Ming Shih, Mei Chun Chen, Ya Hsin Lai, Jun Hao Chen, Chien Ming Chen, Chun Nan Lin

研究成果: 雜誌貢獻文章

25 引文 (Scopus)

摘要

Rhamnus nakaharai Hayata (Rhamnaceae) has been used as a folk medicine in Taiwan for treating constipation, inflammation, tumors, and asthma. 3-O-Methylquercetin (3-MQ), a main constituent of the plant, has been reported to inhibit total cAMP-and cGMP-phosphodiesterase (PDE) of guinea pig trachealis at low concentrations. 3-MQ has been also reported to more selectively inhibit PDE3 than PDE4 with a low Km value. Therefore we were interested in investigating its suppressive effects on ovalbumin (OVA)-induced airway hyperresponsiveness in vivo and in vitro. 3-MQ (3-30 μmol/kg, i.p.) significantly suppressed the enhanced pause (Penh) value induced by aerosolized methacholine (50 mg/mL) in sensitized mice after secondary allergen challenge. 3-MQ (3-30 μmol/kg, i.p.) also significantly suppressed total inflammatory cells, macrophages, neutrophils, and eosinophils, but not lymphocytes. In addition, 3-MQ (3 μmol/kg, i.p.) significantly decreased the secretion of TNF-α, and at the highest dose (30 μmol/kg, i.p.) even decreased the secretions of IL-4, IL-5, and TNF-α. 3-MQ (1-10 μM) as well as Ro 20-1724 (3-30 μM), a selective PDE4 inhibitor, significantly attenuated OVA (100 μg/ mL)-induced contractions. 3-MQ (30 μM) as well as milrinone (1 -10 μM), a selective PDE3 inhibitor, significantly enhanced baseline contractions in isolated guinea pig left and right atria. However, neither 3-MQ nor milrinone significantly affected baseline beating rate in the right atria. 3-MQ (3-30 μmol/kg, i.p.) did not significantly affect systolic pressure in conscious mice. In conclusion, 3-MQ has both anti-inflammatory and bronchodilating effects, and has the potential for use in the treatment of asthma at a dose without affecting blood pressure.
原文英語
頁(從 - 到)1123-1127
頁數5
期刊Planta Medica
70
發行號12
DOIs
出版狀態已發佈 - 十二月 2004

指紋

ovalbumin
Ovalbumin
asthma
guinea pigs
secretion
Rhamnus
Rhamnaceae
interleukin-5
constipation
mice
interleukin-4
eosinophils
dosage
traditional medicine
allergens
blood pressure
neutrophils
Taiwan
macrophages
lymphocytes

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

引用此文

Ko, W-C., Shih, C. M., Chen, M. C., Lai, Y. H., Chen, J. H., Chen, C. M., & Lin, C. N. (2004). Suppressive effects of 3-O-methylquercetin on ovalbumin-induced airway hyperresponsiveness. Planta Medica, 70(12), 1123-1127. https://doi.org/10.1055/s-2004-835838

Suppressive effects of 3-O-methylquercetin on ovalbumin-induced airway hyperresponsiveness. / Ko, Wun-Chang; Shih, Chwen Ming; Chen, Mei Chun; Lai, Ya Hsin; Chen, Jun Hao; Chen, Chien Ming; Lin, Chun Nan.

於: Planta Medica, 卷 70, 編號 12, 12.2004, p. 1123-1127.

研究成果: 雜誌貢獻文章

Ko, W-C, Shih, CM, Chen, MC, Lai, YH, Chen, JH, Chen, CM & Lin, CN 2004, 'Suppressive effects of 3-O-methylquercetin on ovalbumin-induced airway hyperresponsiveness', Planta Medica, 卷 70, 編號 12, 頁 1123-1127. https://doi.org/10.1055/s-2004-835838
Ko, Wun-Chang ; Shih, Chwen Ming ; Chen, Mei Chun ; Lai, Ya Hsin ; Chen, Jun Hao ; Chen, Chien Ming ; Lin, Chun Nan. / Suppressive effects of 3-O-methylquercetin on ovalbumin-induced airway hyperresponsiveness. 於: Planta Medica. 2004 ; 卷 70, 編號 12. 頁 1123-1127.
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abstract = "Rhamnus nakaharai Hayata (Rhamnaceae) has been used as a folk medicine in Taiwan for treating constipation, inflammation, tumors, and asthma. 3-O-Methylquercetin (3-MQ), a main constituent of the plant, has been reported to inhibit total cAMP-and cGMP-phosphodiesterase (PDE) of guinea pig trachealis at low concentrations. 3-MQ has been also reported to more selectively inhibit PDE3 than PDE4 with a low Km value. Therefore we were interested in investigating its suppressive effects on ovalbumin (OVA)-induced airway hyperresponsiveness in vivo and in vitro. 3-MQ (3-30 μmol/kg, i.p.) significantly suppressed the enhanced pause (Penh) value induced by aerosolized methacholine (50 mg/mL) in sensitized mice after secondary allergen challenge. 3-MQ (3-30 μmol/kg, i.p.) also significantly suppressed total inflammatory cells, macrophages, neutrophils, and eosinophils, but not lymphocytes. In addition, 3-MQ (3 μmol/kg, i.p.) significantly decreased the secretion of TNF-α, and at the highest dose (30 μmol/kg, i.p.) even decreased the secretions of IL-4, IL-5, and TNF-α. 3-MQ (1-10 μM) as well as Ro 20-1724 (3-30 μM), a selective PDE4 inhibitor, significantly attenuated OVA (100 μg/ mL)-induced contractions. 3-MQ (30 μM) as well as milrinone (1 -10 μM), a selective PDE3 inhibitor, significantly enhanced baseline contractions in isolated guinea pig left and right atria. However, neither 3-MQ nor milrinone significantly affected baseline beating rate in the right atria. 3-MQ (3-30 μmol/kg, i.p.) did not significantly affect systolic pressure in conscious mice. In conclusion, 3-MQ has both anti-inflammatory and bronchodilating effects, and has the potential for use in the treatment of asthma at a dose without affecting blood pressure.",
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AU - Lai, Ya Hsin

AU - Chen, Jun Hao

AU - Chen, Chien Ming

AU - Lin, Chun Nan

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N2 - Rhamnus nakaharai Hayata (Rhamnaceae) has been used as a folk medicine in Taiwan for treating constipation, inflammation, tumors, and asthma. 3-O-Methylquercetin (3-MQ), a main constituent of the plant, has been reported to inhibit total cAMP-and cGMP-phosphodiesterase (PDE) of guinea pig trachealis at low concentrations. 3-MQ has been also reported to more selectively inhibit PDE3 than PDE4 with a low Km value. Therefore we were interested in investigating its suppressive effects on ovalbumin (OVA)-induced airway hyperresponsiveness in vivo and in vitro. 3-MQ (3-30 μmol/kg, i.p.) significantly suppressed the enhanced pause (Penh) value induced by aerosolized methacholine (50 mg/mL) in sensitized mice after secondary allergen challenge. 3-MQ (3-30 μmol/kg, i.p.) also significantly suppressed total inflammatory cells, macrophages, neutrophils, and eosinophils, but not lymphocytes. In addition, 3-MQ (3 μmol/kg, i.p.) significantly decreased the secretion of TNF-α, and at the highest dose (30 μmol/kg, i.p.) even decreased the secretions of IL-4, IL-5, and TNF-α. 3-MQ (1-10 μM) as well as Ro 20-1724 (3-30 μM), a selective PDE4 inhibitor, significantly attenuated OVA (100 μg/ mL)-induced contractions. 3-MQ (30 μM) as well as milrinone (1 -10 μM), a selective PDE3 inhibitor, significantly enhanced baseline contractions in isolated guinea pig left and right atria. However, neither 3-MQ nor milrinone significantly affected baseline beating rate in the right atria. 3-MQ (3-30 μmol/kg, i.p.) did not significantly affect systolic pressure in conscious mice. In conclusion, 3-MQ has both anti-inflammatory and bronchodilating effects, and has the potential for use in the treatment of asthma at a dose without affecting blood pressure.

AB - Rhamnus nakaharai Hayata (Rhamnaceae) has been used as a folk medicine in Taiwan for treating constipation, inflammation, tumors, and asthma. 3-O-Methylquercetin (3-MQ), a main constituent of the plant, has been reported to inhibit total cAMP-and cGMP-phosphodiesterase (PDE) of guinea pig trachealis at low concentrations. 3-MQ has been also reported to more selectively inhibit PDE3 than PDE4 with a low Km value. Therefore we were interested in investigating its suppressive effects on ovalbumin (OVA)-induced airway hyperresponsiveness in vivo and in vitro. 3-MQ (3-30 μmol/kg, i.p.) significantly suppressed the enhanced pause (Penh) value induced by aerosolized methacholine (50 mg/mL) in sensitized mice after secondary allergen challenge. 3-MQ (3-30 μmol/kg, i.p.) also significantly suppressed total inflammatory cells, macrophages, neutrophils, and eosinophils, but not lymphocytes. In addition, 3-MQ (3 μmol/kg, i.p.) significantly decreased the secretion of TNF-α, and at the highest dose (30 μmol/kg, i.p.) even decreased the secretions of IL-4, IL-5, and TNF-α. 3-MQ (1-10 μM) as well as Ro 20-1724 (3-30 μM), a selective PDE4 inhibitor, significantly attenuated OVA (100 μg/ mL)-induced contractions. 3-MQ (30 μM) as well as milrinone (1 -10 μM), a selective PDE3 inhibitor, significantly enhanced baseline contractions in isolated guinea pig left and right atria. However, neither 3-MQ nor milrinone significantly affected baseline beating rate in the right atria. 3-MQ (3-30 μmol/kg, i.p.) did not significantly affect systolic pressure in conscious mice. In conclusion, 3-MQ has both anti-inflammatory and bronchodilating effects, and has the potential for use in the treatment of asthma at a dose without affecting blood pressure.

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