Suppression of voltage-gated Na+ channels and neuronal excitability by imperatorin

King Chuen Wu, Yi Hung Chen, Ka Shun Cheng, Yueh Hsiung Kuo, Chin Tsang Yang, Kar Lok Wong, Yuan Kun Tu, Paul Chan, Yuk Man Leung

研究成果: 雜誌貢獻文章

13 引文 (Scopus)

摘要

Imperatorin is a naturally occurring furocoumarin compound isolated from plants such as Angelica archangelica and Cnidium monnieri. It has multiple pharmacological effects including anticonvulsant effects. Here we determined the effects of imperatorin on voltage-gated Na+ channels (VGSC) using whole-cell patch clamp techniques in differentiated neuronal NG108-15 cells. We showed that imperatorin inhibited VGSC; such inhibition did not show state-dependence. Imperatorin caused a left shift in the steady-state inactivation curve without affecting activation gating. The inhibition of VGSC by imperatorin displayed a mild frequency-dependence. Imperatorin was also shown to inhibit VGSC and action potential amplitude without affecting voltage-gated K+ channels in rat hippocampal CA1 neurons. In conclusion, our results suggest that imperatorin dampens neuronal excitability by inhibiting VGSC.

原文英語
頁(從 - 到)49-55
頁數7
期刊European Journal of Pharmacology
721
發行號1-3
DOIs
出版狀態已發佈 - 十二月 5 2013

指紋

Angelica archangelica
Cnidium
Voltage-Gated Potassium Channels
Patch-Clamp Techniques
imperatorin
Anticonvulsants
Action Potentials
Pharmacology
Neurons
Furocoumarins

ASJC Scopus subject areas

  • Pharmacology

引用此文

Wu, K. C., Chen, Y. H., Cheng, K. S., Kuo, Y. H., Yang, C. T., Wong, K. L., ... Leung, Y. M. (2013). Suppression of voltage-gated Na+ channels and neuronal excitability by imperatorin. European Journal of Pharmacology, 721(1-3), 49-55. https://doi.org/10.1016/j.ejphar.2013.09.056

Suppression of voltage-gated Na+ channels and neuronal excitability by imperatorin. / Wu, King Chuen; Chen, Yi Hung; Cheng, Ka Shun; Kuo, Yueh Hsiung; Yang, Chin Tsang; Wong, Kar Lok; Tu, Yuan Kun; Chan, Paul; Leung, Yuk Man.

於: European Journal of Pharmacology, 卷 721, 編號 1-3, 05.12.2013, p. 49-55.

研究成果: 雜誌貢獻文章

Wu, KC, Chen, YH, Cheng, KS, Kuo, YH, Yang, CT, Wong, KL, Tu, YK, Chan, P & Leung, YM 2013, 'Suppression of voltage-gated Na+ channels and neuronal excitability by imperatorin', European Journal of Pharmacology, 卷 721, 編號 1-3, 頁 49-55. https://doi.org/10.1016/j.ejphar.2013.09.056
Wu, King Chuen ; Chen, Yi Hung ; Cheng, Ka Shun ; Kuo, Yueh Hsiung ; Yang, Chin Tsang ; Wong, Kar Lok ; Tu, Yuan Kun ; Chan, Paul ; Leung, Yuk Man. / Suppression of voltage-gated Na+ channels and neuronal excitability by imperatorin. 於: European Journal of Pharmacology. 2013 ; 卷 721, 編號 1-3. 頁 49-55.
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AU - Wu, King Chuen

AU - Chen, Yi Hung

AU - Cheng, Ka Shun

AU - Kuo, Yueh Hsiung

AU - Yang, Chin Tsang

AU - Wong, Kar Lok

AU - Tu, Yuan Kun

AU - Chan, Paul

AU - Leung, Yuk Man

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AB - Imperatorin is a naturally occurring furocoumarin compound isolated from plants such as Angelica archangelica and Cnidium monnieri. It has multiple pharmacological effects including anticonvulsant effects. Here we determined the effects of imperatorin on voltage-gated Na+ channels (VGSC) using whole-cell patch clamp techniques in differentiated neuronal NG108-15 cells. We showed that imperatorin inhibited VGSC; such inhibition did not show state-dependence. Imperatorin caused a left shift in the steady-state inactivation curve without affecting activation gating. The inhibition of VGSC by imperatorin displayed a mild frequency-dependence. Imperatorin was also shown to inhibit VGSC and action potential amplitude without affecting voltage-gated K+ channels in rat hippocampal CA1 neurons. In conclusion, our results suggest that imperatorin dampens neuronal excitability by inhibiting VGSC.

KW - Hippocampal CA1 neurons

KW - Imperatorin

KW - NG108-15 cells

KW - Voltage-gated Na channels

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