Suppression of lipopolysaccharide-induced of inducible nitric oxide synthase and cyclooxygenase-2 by Sanguis Draconis, a dragon's blood resin, in RAW 264.7 cells

Cheuk Sing Choy, Chien Ming Hu, Wen Ta Chiu, Carlos Lam, Yih Ting, Shin Han Tsai, Tzu Chien Wang

研究成果: 雜誌貢獻文章

50 引文 (Scopus)

摘要

Sanguis Draconis (SD) is a kind of dragon's blood resin that is obtained from Daemomorops draco (Palmae). It is used in traditional medicine and has shown anti-inflammatory activity in some diseases. In this study, we examined the effects of Sanguis Dranonis ethanol extract (SDEE) on LPS-induced inflammation using RAW 264.7 cells. Our data indicated that SDEE inhibits LPS-stimulated NO, PGE2, IL-1β and TNF-α release, and iNOS and COX-2 expression. Furthermore, SDEE suppressed the LPS-induced p65 expression of NF-κB, which was associated with the inhibition of IκB-α degradation. We also found that the expression of HO-1 was significantly increased in RAW 264.7 cells by SDEE. These results suggest among possibilities of anti-inflammation that SDEE inhibits the production of NO and PGE2 by the down-regulation of iNOS and COX-2 gene expression via the suppression of NF-κB (p65) activation. SDEE can induce HO-1 over-expression in macrophage cells, which indicates that it may possess antioxidant properties. This result means that SEDD its anti-inflammatory effects in macrophages may be through a novel mechanism that involves the action of HO-1. Thus, SD could provide a potential therapeutic approach for inflammation-associated disorders.
原文英語
頁(從 - 到)455-462
頁數8
期刊Journal of Ethnopharmacology
115
發行號3
DOIs
出版狀態已發佈 - 二月 12 2007

指紋

Nitric Oxide Synthase Type II
Cyclooxygenase 2
Lipopolysaccharides
Ethanol
Inflammation
Dinoprostone
Anti-Inflammatory Agents
Macrophages
Traditional Medicine
Interleukin-1
RAW 264.7 Cells
dragon's blood
Down-Regulation
Antioxidants
Gene Expression

ASJC Scopus subject areas

  • Pharmacology

引用此文

Suppression of lipopolysaccharide-induced of inducible nitric oxide synthase and cyclooxygenase-2 by Sanguis Draconis, a dragon's blood resin, in RAW 264.7 cells. / Choy, Cheuk Sing; Hu, Chien Ming; Chiu, Wen Ta; Lam, Carlos; Ting, Yih; Tsai, Shin Han; Wang, Tzu Chien.

於: Journal of Ethnopharmacology, 卷 115, 編號 3, 12.02.2007, p. 455-462.

研究成果: 雜誌貢獻文章

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abstract = "Sanguis Draconis (SD) is a kind of dragon's blood resin that is obtained from Daemomorops draco (Palmae). It is used in traditional medicine and has shown anti-inflammatory activity in some diseases. In this study, we examined the effects of Sanguis Dranonis ethanol extract (SDEE) on LPS-induced inflammation using RAW 264.7 cells. Our data indicated that SDEE inhibits LPS-stimulated NO, PGE2, IL-1β and TNF-α release, and iNOS and COX-2 expression. Furthermore, SDEE suppressed the LPS-induced p65 expression of NF-κB, which was associated with the inhibition of IκB-α degradation. We also found that the expression of HO-1 was significantly increased in RAW 264.7 cells by SDEE. These results suggest among possibilities of anti-inflammation that SDEE inhibits the production of NO and PGE2 by the down-regulation of iNOS and COX-2 gene expression via the suppression of NF-κB (p65) activation. SDEE can induce HO-1 over-expression in macrophage cells, which indicates that it may possess antioxidant properties. This result means that SEDD its anti-inflammatory effects in macrophages may be through a novel mechanism that involves the action of HO-1. Thus, SD could provide a potential therapeutic approach for inflammation-associated disorders.",
keywords = "Cyclooxygenase-2, Inducible nitric oxide synthase, Lipopolysaccharide, Nuclear factor-κB, Sanguis Draconis",
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AU - Choy, Cheuk Sing

AU - Hu, Chien Ming

AU - Chiu, Wen Ta

AU - Lam, Carlos

AU - Ting, Yih

AU - Tsai, Shin Han

AU - Wang, Tzu Chien

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N2 - Sanguis Draconis (SD) is a kind of dragon's blood resin that is obtained from Daemomorops draco (Palmae). It is used in traditional medicine and has shown anti-inflammatory activity in some diseases. In this study, we examined the effects of Sanguis Dranonis ethanol extract (SDEE) on LPS-induced inflammation using RAW 264.7 cells. Our data indicated that SDEE inhibits LPS-stimulated NO, PGE2, IL-1β and TNF-α release, and iNOS and COX-2 expression. Furthermore, SDEE suppressed the LPS-induced p65 expression of NF-κB, which was associated with the inhibition of IκB-α degradation. We also found that the expression of HO-1 was significantly increased in RAW 264.7 cells by SDEE. These results suggest among possibilities of anti-inflammation that SDEE inhibits the production of NO and PGE2 by the down-regulation of iNOS and COX-2 gene expression via the suppression of NF-κB (p65) activation. SDEE can induce HO-1 over-expression in macrophage cells, which indicates that it may possess antioxidant properties. This result means that SEDD its anti-inflammatory effects in macrophages may be through a novel mechanism that involves the action of HO-1. Thus, SD could provide a potential therapeutic approach for inflammation-associated disorders.

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