Suppression of isoproterenol-induced apoptosis in H9c2 cardiomyoblast cells by daidzein through activation of Akt

Wei Syun Hu, Yueh Min Lin, Wei Wen Kuo, Lung Fa Pan, Yu Lan Yeh, Yi Hui Li, Chia Hua Kuo, Ray Jade Chen, V. Vijaya Padma, Tung Sheng Chen, Chih Yang Huang

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5 引文 斯高帕斯(Scopus)

摘要

Increased serum norepinephrine level is one of pathological processes relating to heart disease (HD). Estrogens are considered as potential therapeutics for the treatment of HD; however, estrogen supplementation shows some side-effects, such as increasing the risk of developing breast, endometrial and ovarian cancers. This study investigated the cardio-protective effects of daidzein (Dai), a selective estrogen receptor modulator (SERM) from soy bean extract, in H9c2 cardiomyoblast cells treated with isoproterenol (ISO), a norepinephrine analog. In this in vitro model, H9c2 cells treated with Dai at different concentrations showed no statistical difference in cell viability. TdT-mediated digoxigenin-dUTP nick-end labeling (TUNEL) data and western blotting results indicated that Dai treated-H9c2 cells recovered from the damage induced by ISO. The recovery effects of Dai on ISO-induced damage were blocked by inhibition of Akt activation through adding Akt inhibitor. On the other hand, the fold changes of phosphorylated Akt (p-Akt)/Akt normalized with the control for con, 0.25, 0.5, 1, 3 and 24 h of treatment were 1, 2, 5, 13, 11 and 10, respectively. In conclusion, Dai ameliorates apoptosis of cardiomyoblasts induced by ISO through Akt signaling pathway.
原文英語
頁(從 - 到)323-330
頁數8
期刊Chinese Journal of Physiology
59
發行號6
DOIs
出版狀態已發佈 - 2016

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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