Suppression of inducible nitric oxide synthase and cyclooxygenase-2 in downregulating nuclear factor-kappa B pathway by garcinol

Chiung Ho Liao, Shengmin Sang, Yu Chih Liang, Chi Tang Ho, Jen Kun Lin

研究成果: 雜誌貢獻文章同行評審

88 引文 斯高帕斯(Scopus)

摘要

Garcinol is a polyisoprenylated benzophenone derivative of Garcinia indica fruit rind and other species. Recent studies have demonstrated that garcinol exhibited antioxidative effects in vitro. In this study, we found that garcinol inhibited the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-activated macrophages. Western blot analyzes and gel-shift assays revealed that garcinol strongly blocks the activation of eukaryotic transcription factor nuclear factor-kappa B (NF-κB)-induced by LPS. Moreover, transient transfection experiments showed that garcinol inhibited the NF-κB-dependent transcriptional activity. Based on these data, we demonstrated that inhibition of LPS-induced NF-κB activation occurred through suppressing the phosphorylation of IκBα and p38 mitogen-activated kinase (MAPK). Garcinol also lowers the LPS-induced increase of intracellular reactive oxygen species (ROS), which contributes to the activation of NF-κB. The NF-κB signaling pathway leads to inflammatory reaction and our results suggest that garcinol suppresses the expression of iNOS in this pathway.
原文英語
頁(從 - 到)140-149
頁數10
期刊Molecular Carcinogenesis
41
發行號3
DOIs
出版狀態已發佈 - 11月 2004
對外發佈

ASJC Scopus subject areas

  • 癌症研究
  • 分子生物學

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