Suppression of gene amplification and chromosomal DNA integration by the DNA mismatch repair system

Ching Tai Lin, Yi Lisa Lyu, Hai Xiao, Wei Hsin Lin, Jacqueline Whang-Peng

研究成果: 雜誌貢獻文章

34 引文 斯高帕斯(Scopus)

摘要

Mismatch repair (MMR)-deficient cells are shown to produce > 15-fold more methotrexate-resistant colonies than MMR normal cells. The increased resistance to methotrexate is primarily due to gene amplification since all the resistant clones contain double-minute chromosomes and increased copy numbers of the DHFR gene. In addition, integration of linearized or retroviral DNAs into chromosomes is also significantly elevated in MMR-deficient cells. These results suggest that in addition to microsatellite instability and homeologous recombination, MMR is also involved in suppression of other genome instabilities such as gene amplification and chromosomal DNA integration.
原文英語
頁(從 - 到)3304-3310
頁數7
期刊Nucleic Acids Research
29
發行號16
出版狀態已發佈 - 八月 15 2001
對外發佈Yes

ASJC Scopus subject areas

  • Genetics

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