Suppression of Etk/Bmx protects against ischemic brain injury

Kai Yun Chen, John Chung Che Wu, Cheng Fu Chang, Yuan Hao Chen, Wen Ta Chiu, Ya Hsin Lou, Yen Hua Chen, Hsiu Ming Shih, Yung Hsiao Chiang

研究成果: 雜誌貢獻文章

5 引文 (Scopus)

摘要

Etk/Bmx (epithelial and endothelial tyrosine kinase, also known as BMX), a member of the Tec (tyrosine kinase expressed in hepatocellular carcinoma) family of protein-tyrosine kinases, is an important regulator of signal transduction for the activation of cell growth, differentiation, and development. We have previously reported that activation of Etk leads to apoptosis in MDA-MB-468 cells. The purpose of this study was to examine the role of Etk in neuronal injury induced by H 2O 2 or ischemia. Using Western blot analysis and immunohistochemistry, we found that treatment with H 2O 2 significantly enhanced phosphorylation of Etk and its downstream signaling molecule Stat1 in primary cortical neurons. Inhibiting Etk activity by LFMA13 or knocking down Etk expression by a specific shRNA increased the survival of primary cortical neurons. Similarly, at 1 day after a 60-min middle cerebral artery occlusion (MCAo) in adult rats, both phosphorylated Etk and Stat1 were coexpressed with apoptotic markers in neurons in the penumbra. Pretreatment with LFM-A13 or an adenoviral vector encoding the kinase deletion mutant EtkΔk attenuated caspase- 3 activity and infarct volume in ischemic brain. All together, our data suggest that Etk is activated after neuronal injury. Suppressing Etk activity protects against neurodegeneration in ischemic brain.
原文英語
頁(從 - 到)345-354
頁數10
期刊Cell Transplantation
21
發行號1
DOIs
出版狀態已發佈 - 2012

指紋

Brain Injuries
Neurons
Brain
Protein-Tyrosine Kinases
Chemical activation
Signal transduction
Phosphorylation
Middle Cerebral Artery Infarction
Wounds and Injuries
Cell growth
Cell death
Growth and Development
Caspase 3
Small Interfering RNA
Rats
Cell Differentiation
Hepatocellular Carcinoma
Signal Transduction
Phosphotransferases
Ischemia

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation
  • Biomedical Engineering

引用此文

Suppression of Etk/Bmx protects against ischemic brain injury. / Chen, Kai Yun; Wu, John Chung Che; Chang, Cheng Fu; Chen, Yuan Hao; Chiu, Wen Ta; Lou, Ya Hsin; Chen, Yen Hua; Shih, Hsiu Ming; Chiang, Yung Hsiao.

於: Cell Transplantation, 卷 21, 編號 1, 2012, p. 345-354.

研究成果: 雜誌貢獻文章

Chen, KY, Wu, JCC, Chang, CF, Chen, YH, Chiu, WT, Lou, YH, Chen, YH, Shih, HM & Chiang, YH 2012, 'Suppression of Etk/Bmx protects against ischemic brain injury', Cell Transplantation, 卷 21, 編號 1, 頁 345-354. https://doi.org/10.3727/096368911X582741
Chen, Kai Yun ; Wu, John Chung Che ; Chang, Cheng Fu ; Chen, Yuan Hao ; Chiu, Wen Ta ; Lou, Ya Hsin ; Chen, Yen Hua ; Shih, Hsiu Ming ; Chiang, Yung Hsiao. / Suppression of Etk/Bmx protects against ischemic brain injury. 於: Cell Transplantation. 2012 ; 卷 21, 編號 1. 頁 345-354.
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