Suppression of endotoxin-induced proinflammatory responses by citrus pectin through blocking LPS signaling pathways

Chien Ho Chen, Ming Thau Sheu, Tzeng-Fu Chen, Ying Ching Wang, Wen Chi Hou, Der Zen Liu, Tsao-Chuen Chung, Yu Chih Liang

研究成果: 雜誌貢獻文章同行評審

101 引文 斯高帕斯(Scopus)

摘要

Pectin is composed of complex polysaccharides rich in galactoside residues, and it is most abundant in citrus fruits. Pectin and modified pectin have been found to exhibit anti-mutagenic activity and inhibit cancer metastasis and proliferation, with no evidence of toxicity or other serious side effects. In this study, we investigated the inhibitory effect of pectin at different degrees of esterification (DEs) on the expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-activated macrophages. Western blot and RT-PCR analyses demonstrated that 30% esterified pectin (DE30), DE60 pectin, and DE90 pectin significantly inhibited the protein and mRNA expressions of iNOS and COX-2 in LPS-activated macrophages, and DE90 pectin was the most-potent inhibitor. To clarify the mechanisms involved, DE90 pectin was found to inhibit the phosphorylation of MAPKs and IKK kinase activity. In addition, DE90 pectin inhibited the activation of NF-κB and AP-1 by electrophoretic mobility shift assay and transient transfection experiments. Finally, we found that DE90 pectin could bind with LPS, and might result in decreased binding of LPS to its receptor. These results suggest that modulation of iNOS and COX-2 expressions by dietary pectin may be important in cancer chemoprevention and anti-inflammation.
原文英語
頁(從 - 到)1001-1009
頁數9
期刊Biochemical Pharmacology
72
發行號8
DOIs
出版狀態已發佈 - 十月 16 2006

ASJC Scopus subject areas

  • Pharmacology

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