Suppression effect of seminal vesicle autoantigen on platelet-activating factor-induced mouse sperm capacitation

Yen Hua Huang, Yee Hsiung Chen, Chun Mao Lin, Yi Yun Ciou, Shin Peih Kuo, Chien Tsu Chen, Chwen Ming Shih, E-E Chang

研究成果: 雜誌貢獻文章

12 引文 (Scopus)

摘要

Mammalian sperm gain the ability to fertilize an egg successfully by the capacitation process. An unregulated capacitation process causes sperm to undergo a spontaneous acrosome reaction (AR) and resulting in loss of their fertilization activity. Thus, functional sperm activation is tightly regulated by a capacitation and suppression (decapacitation) mechanism. Factors, such as platelet-activating factor (PAF) present in both sperm and the female genital tract, are able to stimulate sperm capacitation. Seminal plasma is thought to have the ability to suppress sperm capacitation; however, the regulatory mechanisms of seminal plasma protein on sperm capacitation are not well understood. Recently, we demonstrated that seminal vesicle autoantigen (SVA), a major seminal vesicle secretory protein, is able to suppress mouse sperm capacitation. To further study the suppression spectra of SVA on sperm capacitation, we investigated the effect of SVA on PAF-induced mouse sperm capacitation-related signals. Here, we demonstrate that SVA decreases the [Ca2+], to suppress the PAF's effects on [Ca2+] i, the cAMP level, protein tyrosine phosphorylation, and capacitation. The inhibition of PAF-induced protein tyrosine phosphorylation and capacitation by SVA can be reversed by cAMP agonists. Characterization of the interactions of SVA with PAF by TLC overlay and tryptophan fluorescence spectrum analyses indicates that SVA is capable of binding PAF with an apparent dissociation constant Kd>50 μM. Together with these results, we demonstrate that SVA deceases [Ca2+], and cross-talks with PAF-induced intracellular signals to regulate mouse sperm capacitation.
原文英語
頁(從 - 到)941-951
頁數11
期刊Journal of Cellular Biochemistry
100
發行號4
DOIs
出版狀態已發佈 - 三月 1 2007

指紋

Sperm Capacitation
Platelet Activating Factor
Spermatozoa
Phosphorylation
Tyrosine
Seminal Vesicle Secretory Proteins
Seminal Plasma Proteins
Acrosome Reaction
seminal vesicle autoantigen
Semen
Fertilization
Tryptophan
Ovum
Spectrum Analysis
Proteins
Fluorescence
Chemical activation
Plasmas

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

引用此文

Suppression effect of seminal vesicle autoantigen on platelet-activating factor-induced mouse sperm capacitation. / Huang, Yen Hua; Chen, Yee Hsiung; Lin, Chun Mao; Ciou, Yi Yun; Kuo, Shin Peih; Chen, Chien Tsu; Shih, Chwen Ming; Chang, E-E.

於: Journal of Cellular Biochemistry, 卷 100, 編號 4, 01.03.2007, p. 941-951.

研究成果: 雜誌貢獻文章

@article{30f6e56f9f494e4d809440f2e6393cb3,
title = "Suppression effect of seminal vesicle autoantigen on platelet-activating factor-induced mouse sperm capacitation",
abstract = "Mammalian sperm gain the ability to fertilize an egg successfully by the capacitation process. An unregulated capacitation process causes sperm to undergo a spontaneous acrosome reaction (AR) and resulting in loss of their fertilization activity. Thus, functional sperm activation is tightly regulated by a capacitation and suppression (decapacitation) mechanism. Factors, such as platelet-activating factor (PAF) present in both sperm and the female genital tract, are able to stimulate sperm capacitation. Seminal plasma is thought to have the ability to suppress sperm capacitation; however, the regulatory mechanisms of seminal plasma protein on sperm capacitation are not well understood. Recently, we demonstrated that seminal vesicle autoantigen (SVA), a major seminal vesicle secretory protein, is able to suppress mouse sperm capacitation. To further study the suppression spectra of SVA on sperm capacitation, we investigated the effect of SVA on PAF-induced mouse sperm capacitation-related signals. Here, we demonstrate that SVA decreases the [Ca2+], to suppress the PAF's effects on [Ca2+] i, the cAMP level, protein tyrosine phosphorylation, and capacitation. The inhibition of PAF-induced protein tyrosine phosphorylation and capacitation by SVA can be reversed by cAMP agonists. Characterization of the interactions of SVA with PAF by TLC overlay and tryptophan fluorescence spectrum analyses indicates that SVA is capable of binding PAF with an apparent dissociation constant Kd>50 μM. Together with these results, we demonstrate that SVA deceases [Ca2+], and cross-talks with PAF-induced intracellular signals to regulate mouse sperm capacitation.",
keywords = "Capacitation, Seminal plasma protein, Sperm",
author = "Huang, {Yen Hua} and Chen, {Yee Hsiung} and Lin, {Chun Mao} and Ciou, {Yi Yun} and Kuo, {Shin Peih} and Chen, {Chien Tsu} and Shih, {Chwen Ming} and E-E Chang",
year = "2007",
month = "3",
day = "1",
doi = "10.1002/jcb.21050",
language = "English",
volume = "100",
pages = "941--951",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "4",

}

TY - JOUR

T1 - Suppression effect of seminal vesicle autoantigen on platelet-activating factor-induced mouse sperm capacitation

AU - Huang, Yen Hua

AU - Chen, Yee Hsiung

AU - Lin, Chun Mao

AU - Ciou, Yi Yun

AU - Kuo, Shin Peih

AU - Chen, Chien Tsu

AU - Shih, Chwen Ming

AU - Chang, E-E

PY - 2007/3/1

Y1 - 2007/3/1

N2 - Mammalian sperm gain the ability to fertilize an egg successfully by the capacitation process. An unregulated capacitation process causes sperm to undergo a spontaneous acrosome reaction (AR) and resulting in loss of their fertilization activity. Thus, functional sperm activation is tightly regulated by a capacitation and suppression (decapacitation) mechanism. Factors, such as platelet-activating factor (PAF) present in both sperm and the female genital tract, are able to stimulate sperm capacitation. Seminal plasma is thought to have the ability to suppress sperm capacitation; however, the regulatory mechanisms of seminal plasma protein on sperm capacitation are not well understood. Recently, we demonstrated that seminal vesicle autoantigen (SVA), a major seminal vesicle secretory protein, is able to suppress mouse sperm capacitation. To further study the suppression spectra of SVA on sperm capacitation, we investigated the effect of SVA on PAF-induced mouse sperm capacitation-related signals. Here, we demonstrate that SVA decreases the [Ca2+], to suppress the PAF's effects on [Ca2+] i, the cAMP level, protein tyrosine phosphorylation, and capacitation. The inhibition of PAF-induced protein tyrosine phosphorylation and capacitation by SVA can be reversed by cAMP agonists. Characterization of the interactions of SVA with PAF by TLC overlay and tryptophan fluorescence spectrum analyses indicates that SVA is capable of binding PAF with an apparent dissociation constant Kd>50 μM. Together with these results, we demonstrate that SVA deceases [Ca2+], and cross-talks with PAF-induced intracellular signals to regulate mouse sperm capacitation.

AB - Mammalian sperm gain the ability to fertilize an egg successfully by the capacitation process. An unregulated capacitation process causes sperm to undergo a spontaneous acrosome reaction (AR) and resulting in loss of their fertilization activity. Thus, functional sperm activation is tightly regulated by a capacitation and suppression (decapacitation) mechanism. Factors, such as platelet-activating factor (PAF) present in both sperm and the female genital tract, are able to stimulate sperm capacitation. Seminal plasma is thought to have the ability to suppress sperm capacitation; however, the regulatory mechanisms of seminal plasma protein on sperm capacitation are not well understood. Recently, we demonstrated that seminal vesicle autoantigen (SVA), a major seminal vesicle secretory protein, is able to suppress mouse sperm capacitation. To further study the suppression spectra of SVA on sperm capacitation, we investigated the effect of SVA on PAF-induced mouse sperm capacitation-related signals. Here, we demonstrate that SVA decreases the [Ca2+], to suppress the PAF's effects on [Ca2+] i, the cAMP level, protein tyrosine phosphorylation, and capacitation. The inhibition of PAF-induced protein tyrosine phosphorylation and capacitation by SVA can be reversed by cAMP agonists. Characterization of the interactions of SVA with PAF by TLC overlay and tryptophan fluorescence spectrum analyses indicates that SVA is capable of binding PAF with an apparent dissociation constant Kd>50 μM. Together with these results, we demonstrate that SVA deceases [Ca2+], and cross-talks with PAF-induced intracellular signals to regulate mouse sperm capacitation.

KW - Capacitation

KW - Seminal plasma protein

KW - Sperm

UR - http://www.scopus.com/inward/record.url?scp=33847046394&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33847046394&partnerID=8YFLogxK

U2 - 10.1002/jcb.21050

DO - 10.1002/jcb.21050

M3 - Article

C2 - 17131380

AN - SCOPUS:33847046394

VL - 100

SP - 941

EP - 951

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

IS - 4

ER -