Super-paramagnetic iron oxide nanoparticles for use in extrapulmonary tuberculosis diagnosis

C. N. Lee, Y. M. Wang, Wen-FuThomas Lai, T. J. Chen, M. C. Yu, C. L. Fang, F. L. Yu, Yu-Hui Tsai, W. H S Chang, C. S. Zuo, P. F. Renshaw

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21 引文 斯高帕斯(Scopus)

摘要

E149-E157 The limited sensitivity of serological tests for mycobacterial antigens has encouraged the development of a nanoparticle probe specific for the extrapulmonary form of Mycobacterium tuberculosis (Mtb). We developed an innovative probe comprised of super-paramagnetic iron oxide (SPIO) nanoparticles conjugated with Mtb surface antibody (MtbsAb-nanoparticles) to provide ultrasensitive imaging of biomarkers involved in extrapulmonary Mtb infection. MtbsAb-nanoparticles were significantly conjugated with Mtb bacilli. The extent of contrast enhancement reduction on magnetic resonance imaging (MRI) for Mtb and human monocytic THP1 cells was proportional to the concentration of MtbsAb-nanoparticles. When MtbsAb-nanoparticles were intravenously injected into mice bearing Mtb granulomas, the granulomatous site showed a 14-fold greater reduction in signal intensity enhancement on T 2-weighted MR images compared with an opposing site that received PBS injection. Mtb sAb-nanoparticles represent a new non-invasive technology for the diagnosis of extrapulmonary Mtb.
原文英語
期刊Clinical Microbiology and Infection
18
發行號6
DOIs
出版狀態已發佈 - 六月 2012

ASJC Scopus subject areas

  • 微生物學(醫學)
  • 傳染性疾病

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