13C-NMR of the Deoxyribose Sugars in Four DNA Oligonucleotide Duplexes: Assignment and Structural Features

Steven R. LaPlante, Nilo Zanatta, Anna Hakkinen, Andrew H.J. Wang, Philip N. Borer

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18 引文 斯高帕斯(Scopus)

摘要

Natural-abundance 13C-NMR spectra have been obtained for four self-complementary DNA oligonucleotides: [d(TAGCGCTA)]2, [d(GGTATACC)]2, [d(CG)3]2, and [d(TCGCG)]2; this paper focuses on the deoxyribose resonances. Assignments were made by a combination of the two-dimensional protondetected heteronuclear correlation experiment and comparison of 1D spectra, accounting for 31P coupling, base composition, and similarities in chemical shift versus temperature profiles (δ vs T). Large shielding and deshielding of the sugar resonances (between 2.0 and -1.9 ppm) are observed upon thermal dissociation of the duplex. The shapes of the δ vs T profiles correlate strongly with the purine/pyrimidine nature of the base attached at C1′ in these duplexes that have a substantial fraction of residues within alternating purine-pyrimidine sequences. The correlation is primarily associated with changes in the equilibrium distribution of furanose pseudorotational states that may arise in part from the relief of interstrand purine-purine steric clashes.

原文英語
頁(從 - 到)2430-2440
頁數11
期刊Biochemistry
33
發行號9
DOIs
出版狀態已發佈 - 3月 1 1994
對外發佈

ASJC Scopus subject areas

  • 生物化學

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