SUMO modification negatively modulates the transcriptional activity of CREB-binding protein via the recruitment of Daxx

Hong Yi Kuo, Che Chang Chang, Jen Chong Jeng, Hui Mei Hu, Ding Yen Lin, Gerd G. Maul, Roland P S Kwok, Hsiu Ming Shih

研究成果: 雜誌貢獻文章同行評審

94 引文 斯高帕斯(Scopus)

摘要

Small ubiquitin-like modifier (SUMO) modification is emerging as an important control in transcription regulation. Here, we show that CREB-binding protein (CBP), a versatile transcriptional coactivator for numerous transcription factors in response to diverse signaling events, can be modified by SUMO-1 at lysine residues 999, 1034, and 1057 both in vitro and in vivo. Mutation of the SUMO acceptor lysine residues either individually or in combination enhanced CBP transcriptional activity, and expression of a SUMO protease SENP2 potentiated the transcriptional activity of CBP wild-type but not its sumoylation mutant, indicating that SUMO modification negatively regulates CBP transcriptional activity. Furthermore, we demonstrated an interaction of SUMO-1-modified CBP with the transcriptional corepressor Daxx and an essential role of Daxx in mediating SUMO-dependent transcriptional regulation of CBP through histone deacetylase 2 recruitment. Together, our findings indicate that SUMO modification and subsequent recruitment of Daxx represent a previously undescribed mechanism in modulating CBP transcriptional potential.
原文英語
頁(從 - 到)16973-16978
頁數6
期刊Proceedings of the National Academy of Sciences of the United States of America
102
發行號47
DOIs
出版狀態已發佈 - 11月 22 2005
對外發佈

ASJC Scopus subject areas

  • 遺傳學
  • 多學科

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