SUMO modification modulates the transrepression activity of PLZF

Ting Ting Chao, Che Chang Chang, Hsiu Ming Shih

研究成果: 雜誌貢獻文章

17 引文 (Scopus)

摘要

Small ubiquitin-like modifier (SUMO) modification has recently been shown to associate with transcriptional regulation and nuclear body formation. Here, we show that transcription factor PLZF can be SUMO modified at lysine residue 242, 387 and 396. Converting these three SUMO acceptor Lys to Arg 3KR does not significantly affect PLZF nuclear body formation, which is distinct from the scenario of PML sumoylation in PML nuclear body formation. Furthermore, PLZF-3KR markedly reduced the transcriptional repression activity, correlating with a loss of PLZF-mediated growth suppression. These results reveal an important role of SUMO modification in PLZF-mediated transcriptional repression.
原文英語
頁(從 - 到)475-482
頁數8
期刊Biochemical and Biophysical Research Communications
358
發行號2
DOIs
出版狀態已發佈 - 六月 29 2007
對外發佈Yes

指紋

Ubiquitin
Sumoylation
Lysine
Transcription Factors
Growth

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

引用此文

SUMO modification modulates the transrepression activity of PLZF. / Chao, Ting Ting; Chang, Che Chang; Shih, Hsiu Ming.

於: Biochemical and Biophysical Research Communications, 卷 358, 編號 2, 29.06.2007, p. 475-482.

研究成果: 雜誌貢獻文章

@article{37751a9d3bb74ff2a0ea8b8301d202e1,
title = "SUMO modification modulates the transrepression activity of PLZF",
abstract = "Small ubiquitin-like modifier (SUMO) modification has recently been shown to associate with transcriptional regulation and nuclear body formation. Here, we show that transcription factor PLZF can be SUMO modified at lysine residue 242, 387 and 396. Converting these three SUMO acceptor Lys to Arg 3KR does not significantly affect PLZF nuclear body formation, which is distinct from the scenario of PML sumoylation in PML nuclear body formation. Furthermore, PLZF-3KR markedly reduced the transcriptional repression activity, correlating with a loss of PLZF-mediated growth suppression. These results reveal an important role of SUMO modification in PLZF-mediated transcriptional repression.",
keywords = "Nuclear body, PLZF, SUMO modification, Transcriptional repression",
author = "Chao, {Ting Ting} and Chang, {Che Chang} and Shih, {Hsiu Ming}",
year = "2007",
month = "6",
day = "29",
doi = "10.1016/j.bbrc.2007.04.157",
language = "English",
volume = "358",
pages = "475--482",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier B.V.",
number = "2",

}

TY - JOUR

T1 - SUMO modification modulates the transrepression activity of PLZF

AU - Chao, Ting Ting

AU - Chang, Che Chang

AU - Shih, Hsiu Ming

PY - 2007/6/29

Y1 - 2007/6/29

N2 - Small ubiquitin-like modifier (SUMO) modification has recently been shown to associate with transcriptional regulation and nuclear body formation. Here, we show that transcription factor PLZF can be SUMO modified at lysine residue 242, 387 and 396. Converting these three SUMO acceptor Lys to Arg 3KR does not significantly affect PLZF nuclear body formation, which is distinct from the scenario of PML sumoylation in PML nuclear body formation. Furthermore, PLZF-3KR markedly reduced the transcriptional repression activity, correlating with a loss of PLZF-mediated growth suppression. These results reveal an important role of SUMO modification in PLZF-mediated transcriptional repression.

AB - Small ubiquitin-like modifier (SUMO) modification has recently been shown to associate with transcriptional regulation and nuclear body formation. Here, we show that transcription factor PLZF can be SUMO modified at lysine residue 242, 387 and 396. Converting these three SUMO acceptor Lys to Arg 3KR does not significantly affect PLZF nuclear body formation, which is distinct from the scenario of PML sumoylation in PML nuclear body formation. Furthermore, PLZF-3KR markedly reduced the transcriptional repression activity, correlating with a loss of PLZF-mediated growth suppression. These results reveal an important role of SUMO modification in PLZF-mediated transcriptional repression.

KW - Nuclear body

KW - PLZF

KW - SUMO modification

KW - Transcriptional repression

UR - http://www.scopus.com/inward/record.url?scp=34248595692&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34248595692&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2007.04.157

DO - 10.1016/j.bbrc.2007.04.157

M3 - Article

C2 - 17498654

AN - SCOPUS:34248595692

VL - 358

SP - 475

EP - 482

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -