SUMO-1 conjugation to human DNA topoisomerase II isozymes

Yong Mao, Shyamal D. Desai, Leroy F. Liu

研究成果: 雜誌貢獻文章同行評審

123 引文 斯高帕斯(Scopus)


Topoisomerase I-mediated DNA damage induced by camptothecin has been shown to induce rapid small ubiquitin-related modifier (SUMO)-1 conjugation to topoisomerase I. In the current study, we show that topoisomerase II-mediated DNA damage induced by teniposide (VM-26) results in the formation of high molecular weight conjugates of both topoisomerase IIα and IIβ isozymes in HeLa cells. Immunological characterization of these conjugates suggests that both topoisomerase IIα and IIβ isozymes are conjugated to SUMO-1. The involvement of SUMO-1/UBC9 in the modification of topoisomerase II isozymes is also supported by the demonstration of physical interaction between topoisomerase II and SUMO-1/UBC9. Surprisingly, ICRF-193, which does not induce topoisomerase II-mediated DNA damage but traps topoisomerase II into a circular clamp conformation, is also shown to induce similar SUMO-1 conjugation to topoisomerase II isozymes. In addition, we show that both oxidative and heat shock stresses, which can cause protein damage, rapidly increase nuclear SUMO-1 conjugates. These studies raise the question on whether SUMO-1 conjugation to topoisomerases is an indirect result of a DNA damage response or a direct result because of protein conformational changes.

頁(從 - 到)26066-26073
期刊Journal of Biological Chemistry
出版狀態已發佈 - 8月 25 2000

ASJC Scopus subject areas

  • 生物化學
  • 分子生物學
  • 細胞生物學


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