Subcutaneous cysticercosis diagnosed by mitochondrial DNA analysis

Yi Chin Shih, Chien Ming Chen, Yu Huei Huang, Chein Wei Liao, Chia Kwung Fan, Chih Hsun Yang

研究成果: 雜誌貢獻文章

5 引文 (Scopus)

摘要

Background: Cysticercosis is caused by the larval stage of the pork tapeworm, Taenia solium. Central nervous system, eyes, skeletal muscle, and subcutaneous tissue are the most frequent sites of involvement. Methods: We report a case of subcutaneous larval cestode where no diagnostic histological features such as scolex or bladder wall can be seen on serial sections. Identification of the pathogen was attempted using mitochondrial DNA analysis of the histopathologic specimen. Results: Analysis of PCR-amplified DNA sequences targeting T. solium mitochondrial cox1 gene and cob gene confirmed the diagnosis. Conclusions: When characteristic morphology is absent in histopathology specimen, mitochondrial DNA diagnosis is a powerful tool for a definitive diagnosis of cysticercosis. The DNA diagnosis using such histopathologic specimens would also be applicable for identification of other parasites as extensive DNA sequence data are currently available.
原文英語
頁(從 - 到)672-675
頁數4
期刊International Journal of Dermatology
49
發行號6
DOIs
出版狀態已發佈 - 2010
對外發佈Yes

指紋

Cysticercosis
Taenia solium
Mitochondrial DNA
Cestoda
Mitochondrial Genes
Subcutaneous Tissue
Parasites
Skeletal Muscle
Urinary Bladder
Central Nervous System
Muscles
Polymerase Chain Reaction
DNA
Genes

ASJC Scopus subject areas

  • Dermatology
  • Medicine(all)

引用此文

Subcutaneous cysticercosis diagnosed by mitochondrial DNA analysis. / Shih, Yi Chin; Chen, Chien Ming; Huang, Yu Huei; Liao, Chein Wei; Fan, Chia Kwung; Yang, Chih Hsun.

於: International Journal of Dermatology, 卷 49, 編號 6, 2010, p. 672-675.

研究成果: 雜誌貢獻文章

Shih, Yi Chin ; Chen, Chien Ming ; Huang, Yu Huei ; Liao, Chein Wei ; Fan, Chia Kwung ; Yang, Chih Hsun. / Subcutaneous cysticercosis diagnosed by mitochondrial DNA analysis. 於: International Journal of Dermatology. 2010 ; 卷 49, 編號 6. 頁 672-675.
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AU - Chen, Chien Ming

AU - Huang, Yu Huei

AU - Liao, Chein Wei

AU - Fan, Chia Kwung

AU - Yang, Chih Hsun

PY - 2010

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