Structure and recognition of sheared tandem G·A base pairs associated with human centromere DNA sequence at atomic resolution

Yi Gui Gao, Howard Robinson, Ruslan Sanishvili, Andrzej Joachimiak, Andrew H.J. Wang

研究成果: 雜誌貢獻文章同行評審

47 引文 斯高帕斯(Scopus)

摘要

G·A mismatched base pairs are frequently found in nucleic acids. Human centromere DNA sequences contain unusual repeating motifs, e.g., (GAATG)(n)·(CATTC)(n) found in the human chromosome. The purine-rich strand of this repeating pentamer sequence forms duplex and hairpin structures with unusual stability. The high stability of these structures is contributed by the 'sheared' G·A base pairs which present a novel recognition surface for ligands and proteins. We have solved the crystal structure, by the multiple- wavelength anomalous diffraction (MAD) method of d(CCGAATGAGG) in which the centromere core sequence motif GAATG is embedded. Three crystal forms were refined to near-atomic resolution. The structures reveal the detailed conformation of tandem G·A base pairs whose unique hydrogen-bonding surface has interesting interactions with bases, hydrated magnesium ions, cobalt(III)hexaammine, spermine, and water molecules. The results are relevant in understanding the structure associated with human centromere sequence in particular and G·A base pairs in nucleic acids (including RNA, like ribozyme) in general.

原文英語
頁(從 - 到)16452-16460
頁數9
期刊Biochemistry
38
發行號50
DOIs
出版狀態已發佈 - 12月 14 1999
對外發佈

ASJC Scopus subject areas

  • 生物化學

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