Structural requirements for the edema-inducing and hemolytic activities of mastoparan B isolated from the hornet (Vespa basalis) venom

C. L. Ho, Y. L. Lin, W. C. Chen, L. L. Hwang, H. M. Yu, K. T. Wang

研究成果: 雜誌貢獻文章

15 引文 斯高帕斯(Scopus)

摘要

Mastoparan B (MP-B) is a cationic tetradecapeptide isolated from the black-bellied hornet (Vespa basalis) venom. It has a primary structure (LKLKSIVSWAKKVL-CONH2,) distinct from other vespine mastoparans. The peptide caused a dose-dependent swelling in rat hind paw and showed a potent hemolytic activity in guinea pig red blood cells. Studies on the structure activity relationship of the peptide showed that replacing lysine at position 2 (Lys2) by asparagine (Asn) in the MP-B sequence caused about 40% decrease in its edema-inducing activity at 50 μg/paw and 90% decrease in hemolytic activity at 30 μM of the peptide, while the same substitution at Lys4 did not cause a significant change in either activity. Replacing either Lys11 or Lys12 by leucine (Leu) caused little or no decrease in the edema-inducing and hemolytic activities. Decreases in both activities were observed when both Lys11 and Lys12 were replaced by Leu. On the other hand, replacing tryptophan at position 9 (Trp9 by tyrosine or phenylalanine in MP-B sequence almost abolished its hemolytic activity, while the edema-inducing activity was only partially inhibited. Circular dichroism spectra of the peptides measured in 20% trifluoro- ethanol revealed that substitution of Lys and Trp did not cause a significant change in the conformation of MP-B. It appears that Lys2 is crucial for both hemolytic and edema-inducing activities of MP-B, while Trp9 is of special importance to the hemolytic activity of MP-B. Lys11 and Lys12 in MP-B probably play a lesser role in both activities.
原文英語
頁(從 - 到)1027-1035
頁數9
期刊Toxicon
34
發行號9
DOIs
出版狀態已發佈 - 九月 1996
對外發佈Yes

ASJC Scopus subject areas

  • Toxicology

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